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Neurotoxicity Biomarker Assay Development

  • Galina A. Izykenova
  • German A. Khunteev
  • Ivan I. Krasnjuk
  • Vladimir L. Beloborodov
  • Svetlana A. Dambinova
Protocol
Part of the Neuromethods book series (NM, volume 147)

Abstract

Neurotoxicity biomarker concept implying NR2 peptide and antibodies is presented in experimental models of transient cerebral ischemia and intracerebral hematoma. Various platforms of NR2 peptide and antibodies assays are developed to improve diagnostic certainty of cerebral ischemia. The assay bench testing results and methods are described. There are automatic analyzer, ELISA, and point-of-care tests discussed for laboratory and bedside applications.

Key words

Neurotoxicity Biomarkers NR2 peptide Antibodies Immunoassay 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Galina A. Izykenova
    • 1
    • 2
  • German A. Khunteev
    • 1
  • Ivan I. Krasnjuk
    • 2
  • Vladimir L. Beloborodov
    • 4
  • Svetlana A. Dambinova
    • 3
    • 4
  1. 1.CIS Biotech, Inc.AtlantaUSA
  2. 2.Department of Pharmaceutical TechnologyI.M. Sechenov First Moscow State Medical UniversityMoscowRussia
  3. 3.Brain Biomarkers Research Lab Emory Decatur Hospital, Emory HealthcareDecaturUSA
  4. 4.Institute of PharmacyI.M. Sechenov First Moscow State Medical UniversityMoscowRussia

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