Injection and Infusion of Compounds to the Central Nervous System
The present protocol describes in detail the steps necessary for executing two highly versatile and minimally invasive surgical approaches for localized delivery of compounds to the central nervous system. The procedures have been designed for use on laboratory mice but can also be tailored for experimentations involving other small rodent models. Following the instructions outlined below, treatments can either be administered through single injections or infused over a longer period of time, at locations identified through stereotaxic coordinates, which ensure efficient targeting of the brain region of interest, as well as increased reproducibility between surgeries. Although the surgical interventions are well tolerated by laboratory animals, it is recommended to closely monitor the mice postoperatively for a few days, and take the necessary measures to prevent stress and discomfort.
Key wordsIntracranial injection Intraventricular injection Osmotic pump Mouse Central nervous system
- 3.Sztainberg Y, Chen HM, Swann JW, Hao S, Tang B, Wu Z, Tang J, Wan YW, Liu Z, Rigo F, Zoghbi HY (2015) Reversal of phenotypes in MECP2 duplication mice using genetic rescue or antisense oligonucleotides. Nature 528(7580):123–126. https://doi.org/10.1038/nature16159CrossRefPubMedPubMedCentralGoogle Scholar
- 6.Keith BJ, Franklin M, Paxinos G (2008) The mouse brain in stereotaxic coordinates, compact: the coronal plates and diagrams. Elsevier ScienceGoogle Scholar