Isolation and Characterization of Extracellular Vesicles from Mesenchymal Stromal Cells

  • Sally Yunsun Kim
  • Thanh Huyen Phan
  • Christina Limantoro
  • Bill Kalionis
  • Wojciech ChrzanowskiEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2029)


Extracellular vesicles (EVs) have received immense attention in the past decade for their diverse use in diagnosis and therapeutics. Enhancing our understanding of EVs and increasing the reliability and reproducibility of EV research demands the use of standard isolation procedures and multiple characterization methods. Here we describe the most commonly used EV isolation method involving ultracentrifugation, and various characterization methods that include nanoparticle tracking analysis, atomic force microscopy and electron microscopy, which measure the size, concentration, and morphology of EVs.

Key words

Extracellular vesicles Ultracentrifugation Nanoparticle tracking analysis Atomic force microscopy Electron microscopy 



The authors acknowledge The University of Sydney for the SOAR Fellowship for W.Ch. The authors acknowledge the facilities and the scientific and technical assistance of the Bosch Molecular Biology Facility and Australian Microscopy & Microanalysis Research Facility at the Australian Centre for Microscopy & Microanalysis, The University of Sydney.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Sally Yunsun Kim
    • 1
    • 2
  • Thanh Huyen Phan
    • 1
  • Christina Limantoro
    • 1
    • 2
  • Bill Kalionis
    • 3
    • 4
  • Wojciech Chrzanowski
    • 1
    • 2
    Email author
  1. 1.Faculty of Medicine and HealthThe University of Sydney School of PharmacySydneyAustralia
  2. 2.The University of Sydney Nano InstituteSydneyAustralia
  3. 3.Department of Maternal-Fetal Medicine Pregnancy Research CentreUniversity of MelbourneMelbourneAustralia
  4. 4.Department of Obstetrics and GynaecologyRoyal Women’s HospitalParkvilleAustralia

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