Isolation and Characterization of Cardiac Progenitor Cells

  • Parul Dixit
  • Rajesh G. KatareEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2029)


Cardiac progenitor cells (CPCs) are gaining interest as a therapeutic option for the treatment of the heart. Due to the limited pool of CPCs residing in the heart, it is essential to isolate and expand the CPCs in vitro. Here we describe the protocol for isolation and culture of the heterogeneous population of CPCs from right atrial appendage and left ventricular tissue collected from patients undergoing on-pump coronary artery bypass graft surgery for the treatment of ischemic heart disease. Our protocol is developed to simultaneously isolate, culture, and characterize the CPCs from both atrial and ventricular tissues. We also describe the protocol for flow cytometry and immunohistochemical characterization of the isolated CPCs.

Key words

Cardiac progenitor cells Heterogeneous CPC population Isolation and culture Characterization Flow cytometry 


  1. 1.
    Mishra R, Vijayan K, Colletti EJ, Harrington DA, Matthiesen TS, Simpson D, Goh SK, Walker BL, Almeida-Porada G, Wang D, Backer CL, Dudley SC Jr, Wold LE, Kaushal S (2011) Characterization and functionality of cardiac progenitor cells in congenital heart patients. Circulation 123:364–373CrossRefGoogle Scholar
  2. 2.
    Dixit P, Donnelly H, Edamatsu M, Galvin I, Bunton R, Katare R (2017) Progenitor cells from atria, ventricle and peripheral blood of the same patients exhibit functional differences associated with cardiac repair. Int J Cardiol 228:412–421CrossRefGoogle Scholar
  3. 3.
    Herzenberg LA, Tung J, Moore WA, Herzenberg LA, Parks DR (2006) Interpreting flow cytometry data: a guide for the perplexed. Nat Immunol 7:681–685CrossRefGoogle Scholar
  4. 4.
    Davis DR, Kizana E, Terrovitis J, Barth AS, Zhang Y, Smith RR, Miake J, Marban E (2010) Isolation and expansion of functionally-competent cardiac progenitor cells directly from heart biopsies. J Mol Cell Cardiol 49:312–321CrossRefGoogle Scholar
  5. 5.
    Rodier F, Campisi J (2011) Four faces of cellular senescence. J Cell Biol 192:547–556CrossRefGoogle Scholar
  6. 6.
    Toussaint O, Medrano EE, von Zglinicki T (2000) Cellular and molecular mechanisms of stress-induced premature senescence (SIPS) of human diploid fibroblasts and melanocytes. Exp Gerontol 35:927–945CrossRefGoogle Scholar
  7. 7.
    Cummings BS, Schnellmann RG (2004) Measurement of cell death in mammalian cells. Curr Protoc Pharmacol Chapter 12:Unit 12.8PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Physiology-HeartOtagoSchool of Biomedical Sciences, University of OtagoDunedinNew Zealand

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