Isolation and Molecular Characterization of Progenitor Cells from Human Umbilical Cord
Mesenchymal stem cells (MSCs) are multipotent precursor cells which have been isolated from different vascularized tissue sources. Due to their paracrine function of secreting trophic and immunomodulatory molecules, MSCs are successfully used in cell-based transplantations and provide an alternative medical paradigm for treating a variety of devastating disorders. Umbilical cord is a medical waste with a large, readily available donor pool. Since umbilical cord is a fetal tissue, MSCs derived from it are considered more primitive with proliferative and differentiation advantages over adult MSCs. We define here a simple, efficient, and reproducible protocol to isolate MSCs from WJ of human umbilical cord using a nonenzymatic procedure. Under the optimized culture conditions, the WJ-MSCs undergo robust proliferation, can be expanded up to 15–20 passages and express the characteristic MSC surface antigens. They can be differentiated into mesodermal lineages in vitro.
Key wordsWharton’s jelly Mesenchymal stem cells Explant culture Umbilical cord Arteries Flow cytometry
This work was financially supported by SERB, DST, India. We are thankful to Dr. Jayanta Chatterjee, Aastha, Kalyani, for generously providing us with human umbilical cord samples. We thank CSIR and UGC India for the fellowship of Mr. Umesh Goyal and Ms. Ankita Sen, respectively.