Phytochromes pp 193-199 | Cite as

Characterization of Flowering Time Mutants

  • Xu Wang
  • Qing Liu
  • Wenjin He
  • Chentao Lin
  • Qin Wang
Part of the Methods in Molecular Biology book series (MIMB, volume 2026)


Timing of flowering, which is adapted to the ambient environment, is one of the key traits to ensure the reproductive success of plants. Our current understanding of the complex genetic control network of this trait is mostly derived from the studies in the model plant species Arabidopsis thaliana. Arabidopsis thaliana is an annual facultative long-day plant, whose flowering time is controlled by numerous environmental and endogenous factors. Here we briefly summarize the genetic pathways that promote flowering of Arabidopsis and describe standard protocols to characterize the flowering time phenotype of Arabidopsis mutants under laboratory conditions.


Flowering time Flowering pathways Arabidopsis thaliana 



The work in authors’ laboratory is supported by grants of the National Institute of Health (GM56265 to CL), National Science Foundation of China (31500991 to QW) and National Science Foundation of Fujian Province in China (2017J01604 to XW). The authors thank the UCLA-FAFU (Fujian Agriculture and Forestry University) Joint Research Center, Haixia Institute of Science and Technology, and Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology for institutional supports.


  1. 1.
    de Montaigu A, Tóth R, Coupland G (2010) Plant development goes like clockwork. Trends Genet 26:296–306CrossRefGoogle Scholar
  2. 2.
    Jarillo JA, Piñeiro M (2011) Timing is everything in plant development. The central role of floral repressors. Plant Sci 181:364–378CrossRefGoogle Scholar
  3. 3.
    Michaels SD (2009) Flowering time regulation produces much fruit. Curr Opin Plant Biol 12:75–80CrossRefGoogle Scholar
  4. 4.
    Andrés F, Coupland G (2012) The genetic basis of flowering responses to seasonal cues. Nat Rev Genet 13:627–639CrossRefGoogle Scholar
  5. 5.
    Fornara F, de Montaigu A, Coupland G (2010) SnapShot: control of flowering in Arabidopsis. Cell 141:550, 550.e1–2CrossRefGoogle Scholar
  6. 6.
    Wellmer F, Riechmann JL (2010) Gene networks controlling the initiation of flower development. Trends Genet 26:519–527CrossRefGoogle Scholar
  7. 7.
    Hayama R, Coupland G (2004) The molecular basis of diversity in the photoperiodic flowering responses of Arabidopsis and rice. Plant Physiol 135:677–684CrossRefGoogle Scholar
  8. 8.
    Imaizumi T, Kay SA (2006) Photoperiodic control of flowering: not only by coincidence. Trends Plant Sci 11:550–558CrossRefGoogle Scholar
  9. 9.
    Song YH, Shim JS, Kinmonth-Schultz HA, Imaizumi T (2015) Photoperiodic flowering: time measurement mechanisms in leaves. Annu Rev Plant Biol 66:441–464CrossRefGoogle Scholar
  10. 10.
    Johanson U, West J, Lister C, Michaels S, Amasino R, Dean C (2000) Molecular analysis of FRIGIDA, a major determinant of natural variation in Arabidopsis flowering time. Science 290:344–347CrossRefGoogle Scholar
  11. 11.
    Kim D-H, Doyle MR, Sung S, Amasino RM (2009) Vernalization: winter and the timing of flowering in plants. Annu Rev Cell Dev Biol 25:277–299CrossRefGoogle Scholar
  12. 12.
    Michaels SD, He Y, Scortecci KC, Amasino RM (2003) Attenuation of FLOWERING LOCUS C activity as a mechanism for the evolution of summer-annual flowering behavior in Arabidopsis. Proc Natl Acad Sci U S A 100:10102–10107CrossRefGoogle Scholar
  13. 13.
    Song J, Angel A, Howard M, Dean C (2012) Vernalization - a cold-induced epigenetic switch. J Cell Sci 125:3723–3731CrossRefGoogle Scholar
  14. 14.
    Zografos BR, Sung S (2012) Vernalization-mediated chromatin changes. J Exp Bot 63:4343–4348CrossRefGoogle Scholar
  15. 15.
    Wilson RN, Heckman JW, Somerville CR (1992) Gibberellin is required for flowering in Arabidopsis thaliana under short days. Plant Physiol 100:403–408CrossRefGoogle Scholar
  16. 16.
    Harberd NP (2003) Relieving DELLA restraint. Science 299:1853–1854CrossRefGoogle Scholar
  17. 17.
    Schwechheimer C, Willige BC (2009) Shedding light on gibberellic acid signalling. Curr Opin Plant Biol 12:57–62CrossRefGoogle Scholar
  18. 18.
    Kardailsky I, Shukla VK, Ahn JH, Dagenais N, Christensen SK, Nguyen JT, Chory J, Harrison MJ, Weigel D (1999) Activation tagging of the floral inducer FT. Science 286:1962–1965CrossRefGoogle Scholar
  19. 19.
    Lee H, Suh SS, Park E, Cho E, Ahn JH, Kim SG, Lee JS, Kwon YM, Lee I (2000) The AGAMOUS-LIKE 20 MADS domain protein integrates floral inductive pathways in Arabidopsis. Genes Dev 14:2366–2376CrossRefGoogle Scholar
  20. 20.
    Abe M, Kobayashi Y, Yamamoto S, Daimon Y, Yamaguchi A, Ikeda Y, Ichinoki H, Notaguchi M, Goto K, Araki T (2005) FD, a bZIP protein mediating signals from the floral pathway integrator FT at the shoot apex. Science 309:1052–1056CrossRefGoogle Scholar
  21. 21.
    Weigel D, Alvarez J, Smyth DR, Yanofsky MF, Meyerowitz EM (1992) LEAFY controls floral meristem identity in Arabidopsis. Cell 69:843–859CrossRefGoogle Scholar
  22. 22.
    Wigge PA, Kim MC, Jaeger KE, Busch W, Schmid M, Lohmann JU, Weigel D (2005) Integration of spatial and temporal information during floral induction in Arabidopsis. Science 309:1056–1059CrossRefGoogle Scholar
  23. 23.
    Möller-Steinbach Y, Alexandre C, Hennig L (2010) Flowering time control. Methods Mol Biol 655:229–237CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Xu Wang
    • 1
    • 2
  • Qing Liu
    • 1
  • Wenjin He
    • 3
  • Chentao Lin
    • 2
  • Qin Wang
    • 1
    • 2
  1. 1.Basic Forestry and Proteome Research CenterFujian Agriculture and Forestry UniversityFuzhouChina
  2. 2.Department of Molecular, Cell and Developmental BiologyUniversity of CaliforniaLos AngelesUSA
  3. 3.Fujian Normal UniversityFuzhouChina

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