Allergy pp 131-141 | Cite as

Monocyte-Derived Dendritic Cells as Antigen-Presenting Cells in T-Cell Proliferation and Cytokine Production

  • Sun-Sang J. SungEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2020)


Dendritic cells (DC) are the most potent antigen-presenting cells that link innate with adaptive immunity. They circulate the body and sample the microenvironments for maintaining homeostasis and for mounting T-cell responses against invading pathogens, foreign antigens, and aberrant self-proteins. In humans, DC derived from blood monocytes (MDC) by cytokine treatment provide the most abundant and versatile source for studying DC and T-cell biology, and for use as adjuvants in cancer therapy. In asthma patients, T-cell functions are studied by using autologous MDC as accessory cells for allergen presentation. The method for isolating T cells and monocytes from peripheral blood mononuclear cells (PBMC) and the stimulation of T cells to proliferate and produce cytokines by MDC are outlined in this chapter. The method can be applied to the functional studies of T cells and DC in other diseases.

Key words

Monocytes Dendritic cells T lymphocytes Antigen presentation Allergen Asthma Human Peripheral blood mononuclear cells Der p 2 House dust mite 


  1. 1.
    Qu C, Brinck-Jensen NS, Zang M, Chen K (2014) Monocyte-derived dendritic cells: targets as potent antigen-presenting cells for the design of vaccines against infectious diseases. Int J Infect Dis 19:1–5CrossRefGoogle Scholar
  2. 2.
    van de Ven R, Lindenberg JJ, Oosterhoff D, de Gruijl TD (2013) Dendritic cell plasticity in tumor-conditioned skin: CD14 cells at the cross-roads of immune activation and suppression. Front Immunol 4:403PubMedPubMedCentralGoogle Scholar
  3. 3.
    Steinman RM (2007) Dendritic cells: understanding immunogenicity. Eur J Immunol 37:S53–S60CrossRefGoogle Scholar
  4. 4.
    Villadangos JA, Schnorrer P (2007) Intrinsic and cooperative antigen-presenting functions of dendritic-cell subsets in vivo. Nat Rev Immunol 7:543–555. Scholar
  5. 5.
    Dzionek A, Fuchs A, Schmidt P, Cremer S, Zysk M, Miltenyi S et al (2000) BDCA-2, BDCA-3, and BDCA-4: three markers for distinct subsets of dendritic cells in human peripheral blood. J Immunol 165:6037–6046CrossRefGoogle Scholar
  6. 6.
    Bachem A, Guttler S, Hartung E, Ebstein F, Schaefer M, Tannert A et al (2010) Superior antigen cross-presentation and XCR1 expression define human CD11c+CD141+ cells as homologues of mouse CD8+ dendritic cells. J Exp Med 207:1273–1281CrossRefGoogle Scholar
  7. 7.
    Poulin LF, Salio M, Griessinger E, Anjos-Afonso F, Craciun L, Chen JL et al (2010) Characterization of human DNGR-1+ BDCA3+ leukocytes as putative equivalents of mouse CD8alpha+ dendritic cells. J Exp Med 207:1261–1271CrossRefGoogle Scholar
  8. 8.
    Liu K, Nussenzweig MC (2010) Origin and development of dendritic cells. Immunol Rev 234:45–54CrossRefGoogle Scholar
  9. 9.
    Shortman K, Sathe P, Vremec D, Naik S, O’Keeffe M (2013) Plasmacytoid dendritic cell development. Adv Immunol 120:105–126CrossRefGoogle Scholar
  10. 10.
    Cheong C, Matos I, Choi JH, Dandamudi DB, Shrestha E, Longhi MP et al (2010) Microbial stimulation fully differentiates monocytes to DC-SIGN/CD209(+) dendritic cells for immune T cell areas. Cell 143:416–429CrossRefGoogle Scholar
  11. 11.
    Randolph GJ, Beaulieu S, Lebecque S, Steinman RM, Muller WA (1998) Differentiation of monocytes into dendritic cells in a model of transendothelial trafficking. Science 282:480–483CrossRefGoogle Scholar
  12. 12.
    Sallusto F, Lanzavecchia A (1994) Efficient presentation of soluble antigen by cultured human dendritic cells is maintained by granulocyte/macrophage colony-stimulating factor plus interleukin 4 and downregulated by tumor necrosis factor alpha. J Exp Med 179:1109–1118CrossRefGoogle Scholar
  13. 13.
    Dhodapkar MV, Krasovsky J, Steinman RM, Bhardwaj N (2000) Mature dendritic cells boost functionally superior CD8(+) T-cell in humans without foreign helper epitopes. J Clin Invest 105:R9–R14CrossRefGoogle Scholar
  14. 14.
    Thurner B, Haendle I, Roder C, Dieckmann D, Keikavoussi P, Jonuleit H et al (1999) Vaccination with mage-3A1 peptide-pulsed mature, monocyte-derived dendritic cells expands specific cytotoxic T cells and induces regression of some metastases in advanced stage IV melanoma. J Exp Med 190:1669–1678CrossRefGoogle Scholar
  15. 15.
    O’Neill DW, Adams S, Bhardwaj N (2004) Manipulating dendritic cell biology for the active immunotherapy of cancer. Blood 104:2235–2246CrossRefGoogle Scholar
  16. 16.
    Sung SJ, Taketomi EA, Smith AM, Platts-Mills TA, Fu SM (1999) Efficient presentation of house dust mite allergen Der p 2 by monocyte-derived dendritic cells and the role of beta 2 integrins. Scand J Immunol 49:96–105CrossRefGoogle Scholar
  17. 17.
    Rossi M, Young JW (2005) Human dendritic cells: potent antigen-presenting cells at the crossroads of innate and adaptive immunity. J Immunol 175:1373–1381CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Medicine, Center for Immunity, Inflammation, and Regenerative MedicineUniversity of Virginia Health Sciences CenterCharlottesvilleUSA

Personalised recommendations