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Allergy pp 131-141 | Cite as

Monocyte-Derived Dendritic Cells as Antigen-Presenting Cells in T-Cell Proliferation and Cytokine Production

  • Sun-Sang J. SungEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2020)

Abstract

Dendritic cells (DC) are the most potent antigen-presenting cells that link innate with adaptive immunity. They circulate the body and sample the microenvironments for maintaining homeostasis and for mounting T-cell responses against invading pathogens, foreign antigens, and aberrant self-proteins. In humans, DC derived from blood monocytes (MDC) by cytokine treatment provide the most abundant and versatile source for studying DC and T-cell biology, and for use as adjuvants in cancer therapy. In asthma patients, T-cell functions are studied by using autologous MDC as accessory cells for allergen presentation. The method for isolating T cells and monocytes from peripheral blood mononuclear cells (PBMC) and the stimulation of T cells to proliferate and produce cytokines by MDC are outlined in this chapter. The method can be applied to the functional studies of T cells and DC in other diseases.

Key words

Monocytes Dendritic cells T lymphocytes Antigen presentation Allergen Asthma Human Peripheral blood mononuclear cells Der p 2 House dust mite 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Medicine, Center for Immunity, Inflammation, and Regenerative MedicineUniversity of Virginia Health Sciences CenterCharlottesvilleUSA

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