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Transmigration Assays for the Determination of Molecular Interactions Between Hematopoietic Stem Cells and Niche Cells

  • Reinhard HenschlerEmail author
  • Rudolf Richter
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2017)

Abstract

The transmigration capacity of hematopoietic stem and progenitor cells (HSPC) is characteristically associated with their ability to home to sites of hematopoiesis in the transplanted host, to proliferate, to differentiate, and to successfully repopulate the hematopoietic system of a transplanted host. Stimulating agents shown to induce the transmigration of HSPC were often later identified to play significant roles in mobilization of HSPC or their interaction with niche cells in the hematopoietic microenvironment. Transwell migration assays through microporous membranes have been developed in various forms to determine the migration capacity of HSPC or mesenchymal stromal cells (MSCs) toward chemoattractants. We describe here a method of a multi-well reusable transmigration assay using a small volume and low numbers of HSPC, allowing the simple and reproducible determination of HSPC transmigration capacity which enable researchers to obtain rapid answers at limited costs with high reliability.

Key words

Transwell Transmigration Chemoattraction HSPC Chemokines Porous membrane 

Notes

Acknowledgments

We acknowledge the technical contributions by our co-workers Victoria Karpf and Bithiah Grace-Jaganathan.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute of Transfusion MedicineUniversity Hospital LeipzigLeipzigGermany
  2. 2.Department of Internal Medicine, Clinic of ImmunologyHannover Medical SchoolHannoverGermany
  3. 3.Institute of Transfusion Medicine, Blood Donation Service, Bavarian Red CrossRegensburgGermany

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