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Assessment of Young and Aged Hematopoietic Stem Cell Activity by Competitive Serial Transplantation Assays

  • Yu Wei Zhang
  • Nina Cabezas-WallscheidEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2017)

Abstract

Healthy hematopoietic stem cells (HSCs) are capable to self-renew and reconstitute the complete hematopoietic system. Upon aging, there is an increased incidence of blood-related diseases. Age-related phenotypes have been widely studied by bone marrow transplantation experiments, where reconstitution of the transplanted cells is a direct measure of HSC activity. In this protocol we describe a competitive bone marrow transplantation assay to functionally test young and old HSCs.

Key words

Hematopoietic stem cells Transplants Young Aged Bone marrow Competitive 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Max Planck Institute of Immunobiology and EpigeneticsFreiburgGermany
  2. 2.International Max Planck Research School for Molecular and Cellular Biology (IMPRS-MCB)FreiburgGermany

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