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Analysis of the Complement Cascade Activation During Mobilization of Hematopoietic Stem/Progenitor Cells

  • Anna I. Grabowska
  • Jakub M. HawrylukEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2017)

Abstract

It has been shown that the complement cascade is involved in the process of mobilization of hematopoietic stem cells, from their niche in the bone marrow to the peripheral blood. Based on this knowledge modulation of complement, cascade activation may enable the development of better mobilization strategies for poorly mobilizing patients. Herein we present a mobilization protocol in mice model, useful for studying the effect of the complement activation in the mobilization process.

Key words

Mobilization Complement cascade Hematopoietic stem cells G-CSF Innate immunity 

Notes

Acknowledgments

We thank Mateusz Adamiak (Department of Regenerative Medicine and Center for Preclinical Studies and Technology, Warsaw Medical University, Warsaw, Poland) for his helpful suggestions and critical reading of the manuscript and Kamila Bujko (Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, Louisville, KY, USA) for her kind providing of figure representing flow cytometry analysis. We thank Tomasz Wilanowski (Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland) for reviewing the protocol as a native speaker.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Regenerative Medicine, Center for Preclinical Studies and TechnologyWarsaw Medical UniversityWarsawPoland

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