Analysis of the Complement Cascade Activation During Mobilization of Hematopoietic Stem/Progenitor Cells
It has been shown that the complement cascade is involved in the process of mobilization of hematopoietic stem cells, from their niche in the bone marrow to the peripheral blood. Based on this knowledge modulation of complement, cascade activation may enable the development of better mobilization strategies for poorly mobilizing patients. Herein we present a mobilization protocol in mice model, useful for studying the effect of the complement activation in the mobilization process.
Key wordsMobilization Complement cascade Hematopoietic stem cells G-CSF Innate immunity
We thank Mateusz Adamiak (Department of Regenerative Medicine and Center for Preclinical Studies and Technology, Warsaw Medical University, Warsaw, Poland) for his helpful suggestions and critical reading of the manuscript and Kamila Bujko (Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, Louisville, KY, USA) for her kind providing of figure representing flow cytometry analysis. We thank Tomasz Wilanowski (Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland) for reviewing the protocol as a native speaker.
- 1.Ratajczak MZ, Adamiak M, Plonka M, Abdel-Latif A, Ratajczak J (2018) Mobilization of hematopoietic stem cells as a result of innate immunity-mediated sterile inflammation in the bone marrow microenvironment-the involvement of extracellular nucleotides and purinergic signaling. Leukemia 32(5):1116–1123. https://doi.org/10.1038/s41375-018-0087-zCrossRefPubMedPubMedCentralGoogle Scholar
- 4.Reca R, Cramer D, Yan J, Laughlin MJ, Janowska-Wieczorek A, Ratajczak J et al (2007) A novel role of complement in mobilization: immunodeficient mice are poor granulocyte-colony stimulating factor mobilizers because they lack complement-activating immunoglobulins. Stem Cells 25(12):3093–3100CrossRefGoogle Scholar
- 7.Lee HM, Wu W, Wysoczynski M, Liu R, Zuba-Surma EK, Kucia M et al (2009) Impaired mobilization of hematopoietic stem/progenitor cells in C5-deficient mice supports the pivotal involvement of innate immunity in this process and reveals novel promobilization effects of granulocytes. Leukemia 23(11):2052–2062CrossRefGoogle Scholar
- 10.Lee HM, Wysoczynski M, Liu R, Shin DM, Kucia M, Botto M et al (2010) Mobilization studies in complement-deficient mice reveal that optimal AMD3100 mobilization of hematopoietic stem cells depends on complement cascade activation by AMD3100-stimulated granulocytes. Leukemia 24(3):573–582CrossRefGoogle Scholar