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Transposon Mutagenesis of Foodborne Pathogenic Escherichia coli

  • Supraja Puttamreddy
  • F. Chris MinionEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2016)

Abstract

The Enterobacteriaceae, and in particular, Escherichia coli including foodborne pathotypes are particularly amenable to transposon mutagenesis. Here we describe the use of mini-Tn5 and Mu d1(Ap lac) to generate transposon inserts for analysis of enterohemorrhagic Escherichia coli EDL933. We also discuss how to array the library in 96-well plates and sequence individual clones for further analysis.

Key words

Enterohemorrhagic Escherichia coli Transposons Library construction DNA sequence analysis Mu phage 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.MerckAmesUSA
  2. 2.Department of Veterinary Microbiology and Preventive MedicineIowa State UniversityAmesUSA

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