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Construction of a Sequence-Defined Transposon Mutant Library in Staphylococcus aureus

  • Jennifer L. Endres
  • Vijaya Kumar Yajjala
  • Paul D. Fey
  • Kenneth W. BaylesEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2016)

Abstract

Transposon mutagenesis is one of the most widely used strategies to generate a large number of random mutations within a bacterial genome and then to precisely identify the mutated sites. The generation of sequence-defined transposon mutant libraries that are composed of a collection of different mutants, each containing a single transposon insertion mutation within nearly all of the nonessential genes within the genome, is a rapid and reliable way to enhance the study of gene function. In this chapter, we describe the process to generate a sequence-defined transposon mutant library in Staphylococcus aureus utilizing the mariner-based bursa aurealis transposon.

Key words

Staphylococcus aureus Transposon Random mutagenesis bursa aurealis 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Jennifer L. Endres
    • 1
  • Vijaya Kumar Yajjala
    • 1
  • Paul D. Fey
    • 1
  • Kenneth W. Bayles
    • 1
    Email author
  1. 1.Department of Pathology and Microbiology, Center for Staphylococcal ResearchUniversity of Nebraska Medical CenterOmahaUSA

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