Construction of DNA Barcode-Tagged Salmonella Strains
This chapter provides a detailed protocol for construction of DNA barcode-tagged isogenic strains of Salmonella. The protocol is illustrated with S. Enteritidis in this chapter. However, this protocol should be widely applicable to other Salmonella serotypes. A series of the DNA barcode-tagged strains thus constructed can be used in combination with next generation sequencing or quantitative PCR to study the population dynamics of the bacterial pathogen during infection within the host or transmission within a population of the host in a quantitative manner.
Key wordsSalmonella Population dynamics DNA barcode-tagging Overlapping extension PCR Red recombination system Next generation sequencing
- 5.Yang Y, Tellez G, Latorre JD, Ray PM, Hernandez X, Hargis BM, Ricke SC, Kwon YM (2018) Salmonella excludes Salmonella in poultry: confirming an old paradigm using conventional and barcode-tagging approaches. Front Vet Sci 5:101. https://doi.org/10.3389/fvets.2018.00101 CrossRefPubMedPubMedCentralGoogle Scholar