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Protocols of Conjugative Plasmid Transfer in Salmonella: Plate, Broth, and Filter Mating Approaches

  • Bijay K. KhajanchiEmail author
  • Pravin R. Kaldhone
  • Steven L. Foley
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2016)

Abstract

Bacterial conjugation is a natural process that allows for horizontal transmission of DNA from one bacterium to another. Several plasmids carry transposons that encode multiple antimicrobial and metal resistance genes. Conjugative plasmid transfer requires intimate cell-to-cell contacts between the donor and the recipient. Self-conjugative plasmids harbor tra genes which facilitate plasmid transfer from donor to recipient bacterial strain. Here we describe different methods of conjugative plasmid transfers via conjugation.

Key words

Conjugation Plasmid Salmonella tra genes Transposons 

Notes

Acknowledgments

The authors would like to thank Drs. Ashraf Khan and Jing Han for their insightful review of the book chapter. The opinions expressed in this book chapter are solely the responsibility of the authors and do not necessarily represent the official views and policy of the US Food and Drug Administration. Reference to any commercial materials, equipment, or process does not in any way constitute approval, endorsement, or recommendation by the Food and Drug Administration.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Bijay K. Khajanchi
    • 1
    Email author
  • Pravin R. Kaldhone
    • 1
  • Steven L. Foley
    • 1
  1. 1.U.S. Food and Drug AdministrationNational Center for Toxicological ResearchJeffersonUSA

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