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Transposon Mutagenesis of Bacteroides fragilis

  • Yaligara Veeranagouda
  • Fasahath Husain
  • Hannah M. Wexler
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2016)

Abstract

Bacteroides fragilis is Gram-negative obligatory anaerobe which usually resides in the gut of humans and animals. As an important member of the human gut microbiota it plays a vital role in digestion and absorption of nutrients as well as shaping of host immune system. B. fragilis is also infamous for causing serious infections. Treatment of B. fragilis infections caused emergence of multidrug-resistant strains. Molecular biology tools such as transposon mutagenesis help to decipher and understand commensal and pathogenic faces of B. fragilis. Using two mariner transposon vectors we describe the detailed methodology for the transposon mutagenesis of B. fragilis. We also describe two methods for the identification of transposon integration site (TIS) in transposon mutants. Transposon mutagenesis methods described in this chapter serve as a great tool for studying B. fragilis.

Key words

Transposon mutagenesis Bacteroides fragilis Mariner transposon Mutants 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Yaligara Veeranagouda
    • 1
    • 2
  • Fasahath Husain
    • 1
  • Hannah M. Wexler
    • 1
    • 2
  1. 1.Greater Los Angeles VA Health Care SystemLos AngelesUSA
  2. 2.David Geffen School of MedicineUniversity of California Los AngelesLos AngelesUSA

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