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Phloem pp 95-108 | Cite as

Live-Cell Imaging of Fluorescently Tagged Phloem Proteins with Confocal Microscopy

  • Thibaud Cayla
  • Rozenn Le Hir
  • Sylvie DinantEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2014)

Abstract

Confocal laser scanning microscopy can enable observation of phloem cells in living tissues. Here we describe live imaging of phloem cells in the leaves and roots of Arabidopsis thaliana using fluorescently tagged proteins, either expressed in the vasculature using phloem specific promoters or constitutively expressed reference marker lines. Now, the majority of phloem cell types can be identified, allowing a precise cellular and subcellular localization of phloem proteins.

Key words

Fluorescent markers Mobile symplasmic probes Green fluorescent proteins Live-cell imaging Microscopy 

Abbreviations

CC

Companion cell

CFP

Cyan fluorescent protein

GFP

Green fluorescent protein

PPC

Phloem parenchyma cell

SE

Sieve element

YFP

Yellow fluorescent protein

Notes

Acknowledgments

The IJPB benefits from the support of the LabEx Saclay Plant Sciences-SPS (ANR-10-LABX-0040-SPS) managed by the French National Research Agency under an “Investments for the Future” program (ref. ANR-11-IDEX-0003-02). We thank the Imaging and Cytology platform of the Plant Observatory (IJPB Institute, INRA Versailles-Grignon, France) for light microscopy observations. TC was supported by a PhD fellowship from Doctoral School ED145 “Sciences du Végétal,” Paris—Sud XI University.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.UMR 1318, Institut Jean-Pierre Bourgin, INRA-AgroParisTech, CNRS, Université Paris-SaclayVersailles CedexFrance

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