Advertisement

Phloem pp 421-431 | Cite as

Identification of Phloem Mobile mRNAs Using the Solanaceae Heterograft System

  • Chao Xia
  • Yi Zheng
  • Jing Huang
  • Zhangjun Fei
  • Cankui ZhangEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2014)

Abstract

Large numbers of mRNAs move in the phloem and some may function as signals to exert important physiological functions in the distal recipient organs. Generating an authentic list of phloem mobile mRNA is a prerequisite for elucidating their physiological functions. Nicotiana benthamiana can be used as a scion to graft on a tomato (Solanum lycopersicum) rootstock. Thereby, shoot-to-root mobile N. benthamiana mRNAs transported via the phloem can be identified from the root of the tomato rootstock. Due to the close relationship and similar genome sequences of the two species, stringent informatics procedures should be applied to avoid false identification. This heterograft system can be used to study physiological processes associated with mRNAs that are mobile under either normal or adverse growth condition.

Key words

Phloem transport Heterograft Mobile mRNA Systemic signaling RNA-Seq Long-distance transport 

Notes

Acknowledgments

This work was supported by funds from Purdue University as part of AgSEED Crossroads funding to support Indiana’s Agriculture and Rural Development (to C. Z.), National Key Research and Development Project of China (2016YFD0101206), and the US National Science Foundation (IOS-1339287 and IOS-1539831).

References

  1. 1.
    Turnbull CG, Lopez-Cobollo RM (2013) Heavy traffic in the fast lane: long-distance signalling by macromolecules. New Phytol 198:33–51CrossRefGoogle Scholar
  2. 2.
    Spiegelman Z, Golan G, Wolf S (2013) Don’t kill the messenger: long-distance trafficking of mRNA molecules. Plant Sci 213:1–8CrossRefGoogle Scholar
  3. 3.
    Banerjee AK et al (2006) Dynamics of a mobile RNA of potato involved in a long-distance signaling pathway. Plant Cell 18:3443–3457CrossRefGoogle Scholar
  4. 4.
    Turgeon R, Wolf S (2009) Phloem transport: cellular pathways and molecular trafficking. Annu Rev Plant Biol 60:207–221CrossRefGoogle Scholar
  5. 5.
    Kim M et al (2001) Developmental changes due to long-distance movement of a homeobox fusion transcript in tomato. Science 293:287–289CrossRefGoogle Scholar
  6. 6.
    Notaguchi M, Wolf S, Lucas WJ (2012) Phloem-mobile Aux/IAA transcripts target to the root tip and modify root architecture. J Integr Plant Biol 54:760–772CrossRefGoogle Scholar
  7. 7.
    Thompson GA, van Bel AJE (2013) Phloem: molecular cell biology, systemic communication, biotic interactions. Wiley, OxfordGoogle Scholar
  8. 8.
    Thieme CJ et al (2015) Endogenous Arabidopsis messenger RNAs transported to distant tissues. Nat Plants 1:15025CrossRefGoogle Scholar
  9. 9.
    Yang Y et al (2015) Messenger RNA exchange between scions and rootstocks in grafted grapevines. BMC Plant Biol 15:251CrossRefGoogle Scholar
  10. 10.
    Zhang Z et al (2016) Vascular-mediated signalling involved in early phosphate stress response in plants. Nat Plants 2:16033CrossRefGoogle Scholar
  11. 11.
    Xia C et al (2018) Elucidation of the mechanisms of long-distance mRNA movement in a Nicotiana benthamiana/tomato heterograft system. Plant Physiol 177:745–758CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Chao Xia
    • 1
    • 2
  • Yi Zheng
    • 3
  • Jing Huang
    • 2
  • Zhangjun Fei
    • 3
    • 4
  • Cankui Zhang
    • 5
    Email author
  1. 1.Maize Research Institute, Sichuan Agricultural UniversityChengduChina
  2. 2.Department of Agronomy and Purdue Center for Plant BiologyPurdue UniversityWest LafayetteUSA
  3. 3.Boyce Thompson Institute, Cornell UniversityIthacaUSA
  4. 4.U.S. Department of Agriculture-Agricultural Research Service Robert W. Holley Center for Agriculture and HealthIthacaUSA
  5. 5.Department of Agronomy and Purdue Center for Plant BiologyPurdue UniversityWest LafayetteUSA

Personalised recommendations