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Phloem pp 411-420 | Cite as

Efficient Establishment of Interfamily Heterograft of Nicotiana benthamiana and Arabidopsis thaliana

  • Koji Okayasu
  • Michitaka NotaguchiEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2014)

Abstract

The grafting technique has been applied to study systemic signaling in plants, especially to investigate whether gene action is graft transmissible and/or gene products such as RNAs and proteins are transported systemically. Here we describe an interfamily heterograft system between Nicotiana benthamiana scion plants and Arabidopsis stock plants for the identification of systemic phloem-mobile signals. Since these plants belong to evolutionary distant families and genome databases are available for both, we can reliably identify mobile substances transported from one to the other plant.

Key words

Grafting Interfamily grafting Heterograft Wedge-graft Nicotiana Arabidopsis Mobile signals Phloem transport 

Notes

Acknowledgments

We thank Dr. Johannes Liesche for careful editing of the manuscript. This work was supported by JSPS grant 18H04778; JST PRESTO grant JPMJPR15O3; MAFF Science and Technology Research Promotion Program for agriculture, forestry, fisheries and food industry grant 16770567; and MEXT grant 16811669 to M.N.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Graduate School of Bioagricultural SciencesNagoya UniversityNagoyaJapan

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