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Phloem pp 291-299 | Cite as

Using Aphids to Measure Turgor Pressure Inside Sieve Elements

  • Nick GouldEmail author
  • Peter E. H. Minchin
  • Michael R. Thorpe
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2014)

Abstract

The phloem is the long-distance transport system for photoassimilates within the plant. The vulnerability of the phloem tissue to blockage and damage makes it technically difficult to study, which has made it one of the least understood tissues. Transport of solution through the phloem appears to be by osmotically driven bulk flow, making an understanding of phloem hydrostatic pressure important in our comprehension of phloem flow mechanics. Here we describe a method of making in vivo direct transient measurements of phloem hydrostatic pressure using excised aphid stylets to directly access the phloem.

Key words

Aphid stylectomy Hydrostatic pressure Phloem Phloem pressure probe 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Nick Gould
    • 1
    Email author
  • Peter E. H. Minchin
    • 2
  • Michael R. Thorpe
    • 3
  1. 1.The New Zealand Institute for Plant and Food Research LimitedTe PukeNew Zealand
  2. 2.New Zealand Institute for Plant and Food ResearchMotueka Research CentreMotuekaNew Zealand
  3. 3.Plant Science DivisionResearch School of Biology, The Australian National UniversityCanberraAustralia

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