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Phloem pp 195-201 | Cite as

Measuring Phloem Transport Velocity in Arabidopsis Seedlings Using the Fluorescent Coumarin Glucoside, Esculin

  • Kirsten KnoxEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2014)

Abstract

Historically, the ability to measure the velocity of phloem sap in small seedlings and plants has been technically challenging. The phloem tissues are delicate, often flow is blocked entirely if perturbed. Furthermore, the depth that phloem sieve tubes are located within the plant has hindered many techniques. Previously published methods have lacked the spatial and temporal resolution required for measurements in small seedlings, are usually laborious or are not suited to in vivo studies. Here we describe a rapid, high-throughput method using the fluorescent coumarin glucoside esculin as a probe to measure the phloem transport velocity in the roots of young Arabidopsis seedlings

Key words

Esculin Phloem transport velocity Fluorescence Phloem Sucrose 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute of Molecular Plant Science, School of Biological SciencesUniversity of EdinburghEdinburghUK

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