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Phloem pp 177-194 | Cite as

In Vivo Veritas: Radiotracers in Studies of Phloem Transport of Carbohydrate

  • Michael R. ThorpeEmail author
  • Peter E. H. Minchin
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2014)

Abstract

Opportunities and challenges in the use of radiotracers to measure phloem transport are discussed, with an emphasis on noninvasive techniques to trace photoassimilate, carbon’s short-lived isotope 11C, and an eye to pitfalls and traps to avoid. We discuss in turn the rationale for using tracers, the limitations and complications with using short-lived radiotracers like 11C, the physics of decay and detection that need to be known when data are interpreted, and methods of analysis.

Key words

111418Radiotracers Carbon-11 Carbon-14 Radiocarbon Carbon allocation 

References

  1. 1.
    Ross-Elliott TJ, Jensen KH, Haaning KS, Wager BM, Knoblauch J, Howell AH, Mullendore DL, Monteith AG, Paultre D, Yan D, Otero-Perez S, Bourdon M, Sager R, Lee J-Y, Helariutta Y, Knoblauch M, Oparka KJ (2017) Phloem unloading in Arabidopsis roots is convective and regulated by the phloem-pole pericycle. eLife 6:e24125.  https://doi.org/10.7554/eLife.24125CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    Ohkubo Y, Tanaka M, Tabata R, Ogawa-Ohnishi M, Matsubayashi Y (2017) Shoot-to-root mobile polypeptides involved in systemic regulation of nitrogen acquisition. Nat Plants 3:17029.  https://doi.org/10.1038/nplants.2017.29CrossRefPubMedGoogle Scholar
  3. 3.
    Windt CW, Vergeldt FJ, De Jager PA, van As H (2006) MRI of long-distance water transport: a comparison of the phloem and xylem flow characteristics and dynamics in poplar, castor bean, tomato and tobacco. Plant Cell Environ 29:1715–1729. https://doi.org/10.1111/j.1365-3040.2006.01544.xCrossRefGoogle Scholar
  4. 4.
    Plain C, Gerant D, Maillard P, Dannoura M, Dong Y, Zeller B, Priault P, Parent F, Epron D (2009) Tracing of recently assimilated carbon in respiration at high temporal resolution in the field with a tuneable diode laser absorption spectrometer after in situ 13CO2 pulse labelling of 20-year-old beech trees. Tree Physiol 29:1433–1445CrossRefGoogle Scholar
  5. 5.
    Pate JS, Shedley E, Arthur DJ, Admas M (1998) Spatial and temporal variations in phloem sap composition of plantation-grown Eucalyptus globulus. Oecologia 117(8–9):312–322CrossRefGoogle Scholar
  6. 6.
    Kallarackal J, Bauer SN, Nowak H, Hajirezaei MR, Komor E (2012) Diurnal changes in assimilate concentrations and fluxes in the phloem of castor bean (Ricinus communis L.) and tansy (Tanacetum vulgare L.). Planta 236(1):209–223.  https://doi.org/10.1007/s00425-012-1600-7CrossRefPubMedGoogle Scholar
  7. 7.
    Högberg P, Högberg M, Göttlicher S, Betson N, Keel S, Metcalfe D, Campbell C, Schindlbacher A, Hurry V, Lundmark T (2008) High temporal resolution tracing of photosynthate carbon from the tree canopy to forest soil microorganisms. New Phytol 177(1):220–228Google Scholar
  8. 8.
    Dannoura M et al (2011) In situ assessment of the velocity of carbon transfer by tracing 13C in trunk CO2 efflux after pulse labelling: variations among tree species and seasons. New Phytol 190(1):181–192CrossRefGoogle Scholar
  9. 9.
    Giaquinta R, Lin W, Sadler N, Franceschi V (1983) Pathway of phloem unloading in corn roots. Plant Physiol 72:362–367CrossRefGoogle Scholar
  10. 10.
    Minchin PEH, Thorpe MR (1989) Carbon partitioning to whole versus surgically modified ovules of pea: an application of the in vivo measurement of carbon flows over many hours using the short-lived isotope carbon-11. J Exp Bot 40(7):781–787.  https://doi.org/10.1093/jxb/40.7.781CrossRefGoogle Scholar
  11. 11.
    Thorpe MR, Walsh KB, Minchin PEH (1998) Photoassimilate partitioning in nodulated soybean I. 11C methodology. J Exp Bot 49(328):1805–1815CrossRefGoogle Scholar
  12. 12.
    Minchin PEH, McNaughton GS (1987) Xylem transport of recently fixed carbon within Lupin. Aust J Plant Physiol 14(3):325–329Google Scholar
  13. 13.
    Ferrieri AP, Appel H, Ferrieri RA, Schultz JC (2012) Novel application of 2-[18F]fluoro-2-deoxy-d-glucose to study plant defenses. Nucl Med Biol 39(8):1152–1160.  https://doi.org/10.1016/j.nucmedbio.2012.06.005CrossRefPubMedGoogle Scholar
  14. 14.
    Liu DD, Chao WM, Turgeon R (2012) Transport of sucrose, not hexose, in the phloem. J Exp Bot 63:4315–4320CrossRefGoogle Scholar
  15. 15.
    Tran TM, Hampton CS, Brossard TW, Harmata M, Robertson JD, Jurisson SS, Braun DM (2017) In vivo transport of three radioactive [18F]-fluorinated deoxysucrose analogs by the maize sucrose transporter ZmSUT1. Plant Physiol Biochem 115:1–11.  https://doi.org/10.1016/j.plaphy.2017.03.006CrossRefPubMedGoogle Scholar
  16. 16.
    Minchin PEH, Thorpe MR (1987) Measurement of unloading and reloading of photo-assimilate within the stem of bean. J Exp Bot 38:211–220CrossRefGoogle Scholar
  17. 17.
    Minchin PEH, Ryan KG, Thorpe MR (1984) Further evidence of apoplastic unloading into the stem of bean: identification of the phloem buffering pool. J Exp Bot 35(12):1744–1753.  https://doi.org/10.1093/jxb/35.12.1744CrossRefGoogle Scholar
  18. 18.
    Epron D, Cabral OMR, Laclau J-P, Dannoura M, Packer AP, Plain C, Battie-Laclau P, Moreira MZ, Trivelin PCO, Bouillet J-P, Gérant D, Nouvellon Y (2016) In situ 13CO2 pulse labelling of field-grown eucalypt trees revealed the effects of potassium nutrition and throughfall exclusion on phloem transport of photosynthetic carbon. Tree Physiol 36(1):6–21.  https://doi.org/10.1093/treephys/tpv090CrossRefGoogle Scholar
  19. 19.
    Roeb G, Britz SJ (1991) Short-term fluctuations in the transport of assimilates to the ear of wheat measured with steady-state 11C-CO2-labelling of the flag leaf. J Exp Bot 42(4):469–475.  https://doi.org/10.1093/jxb/42.4.469CrossRefGoogle Scholar
  20. 20.
    Goeschl JD, Magnuson CE, Fares Y, Jaeger CH, Nelson CE, Strain BR (1984) Spontaneous and induced blocking and unblocking of phloem transport. Plant Cell Environ 7(8):607–613Google Scholar
  21. 21.
    Hubbell J, Seltzer S (2004) Tables of X-ray mass attenuation coefficients and mass energy-absorption coefficients (version 1.4). National Institute of Standards and Technology, Gaithersburg, MD, USA. http://physics.nist.gov/xaamdi. Accessed 21 Sept 2018
  22. 22.
    Moorby J, Jarman P (1975) The use of compartmental analysis in the study of the movement of carbon through leaves. Planta 122:155–168CrossRefGoogle Scholar
  23. 23.
    Farrar S, Farrar J (1986) Compartmentation and fluxes of sucrose in intact leaf blades of barley. New Phytol 103(4):645–657CrossRefGoogle Scholar
  24. 24.
    Geiger D, Swanson G (1965) Sucrose translocation in sugar beet. Plant Physiol 40:685–690CrossRefGoogle Scholar
  25. 25.
    Babst BA, Ferrieri RA, Gray DW, Lerdau M, Schlyer DJ, Schueller M, Thorpe MR, Orians CM (2005) Jasmonic acid induces rapid changes in carbon transport and partitioning in Populus. New Phytol 167(1):63–72.  https://doi.org/10.1111/j.1469-8137.2005.01388.xCrossRefGoogle Scholar
  26. 26.
    Schmidt L, Hummel GM, Thiele B, Schurr U, Thorpe MR (2015) Leaf wounding or simulated herbivory in young N. attenuata plants reduces carbon delivery to roots and root tips. Planta 241:917–928.  https://doi.org/10.1007/s00425-014-2230-zCrossRefPubMedGoogle Scholar
  27. 27.
    Black MZ, Minchin PH, Gould N, Patterson KJ, Clearwater MJ (2012) Measurement of Bremsstrahlung radiation for in vivo monitoring of 14C tracer distribution between fruit and roots of kiwifruit (Actinidia arguta) cuttings. Planta 236(4):1327–1337.  https://doi.org/10.1007/s00425-012-1685-zCrossRefGoogle Scholar
  28. 28.
    Minchin PEH, Thorpe MR (1996) A method for monitoring γ-radiation from an extended source with uniform sensitivity. Appl Radiat Isot 47:693–696CrossRefGoogle Scholar
  29. 29.
    Pritchard J, Tomos AD, Farrar JF, Minchin PEH, Gould N, Paul MJ, MacRae EA, Ferrieri RA, Gray DW, Thorpe MR (2004) Turgor, solute import and growth in maize roots treated with galactose. Funct Plant Biol 31:1095–1103.  https://doi.org/10.1071/FP04082CrossRefGoogle Scholar
  30. 30.
    Levin C, Hoffman E (1999) Calculation of positron range and its effect on the fundamental limit of positron emission tomography system spatial resolution. Phys Med Biol 44:781–799CrossRefGoogle Scholar
  31. 31.
    De Schepper V, Bühler J, Thorpe M, Roeb G, Huber G, van Dusschoten D, Jahnke S, Steppe K (2013) 11C-PET imaging reveals transport dynamics and sectorial plasticity of oak phloem after girdling. Front Plant Sci 4:200.  https://doi.org/10.3389/fpls.2013.00200CrossRefPubMedPubMedCentralGoogle Scholar
  32. 32.
    Karve AA, Alexoff D, Kim D, Schueller MJ, Ferrieri RA, Babst BA (2015) In vivo quantitative imaging of photoassimilate transport dynamics and allocation in large plants using a commercial positron emission tomography (PET) scanner. BMC Plant Biol 15:273.  https://doi.org/10.1186/s12870-015-0658-3CrossRefPubMedPubMedCentralGoogle Scholar
  33. 33.
    Alexoff DL, Dewey SL, Vaska P, Krishnamoorthy S, Ferrieri R, Schueller M, Schlyer DJ, Fowler JS (2011) PET imaging of thin objects: measuring the effects of positron range and partial-volume averaging in the leaf of Nicotiana tabacum. Nucl Med Biol 38(2):191–200.  https://doi.org/10.1016/j.nucmedbio.2010.08.004CrossRefGoogle Scholar
  34. 34.
    Bühler J, Huber G, Schmid F, Blümler P (2011) Analytical model for long-distance tracer-transport in plants. J Theor Biol 270:70–79. https://doi.org/10.1016/j.jtbi.2010.11.005CrossRefGoogle Scholar
  35. 35.
    Evans NTS, Ebert M, Moorby J (1963) A model for the translocation of the photosynthesis in the soybean. J Exp Bot 14:221–231CrossRefGoogle Scholar
  36. 36.
    Bühler J, von Lieres E, Huber G (2014) A class of compartmental models for long-distance tracer transport in plants. J Theor Biol 341:131–142. https://doi.org/10.1016/j.jtbi.2013.09.023CrossRefGoogle Scholar
  37. 37.
    Minchin PEH, Thorpe MR (2003) Using the short-lived isotope C-11 in mechanistic studies of photosynthate transport. Funct Plant Biol 30(8):831–841.  https://doi.org/10.1071/FP03008CrossRefGoogle Scholar
  38. 38.
    Pickard WF, Minchin PEH, Thorpe MR (1993) Leaf export and partitioning changes induced by short-term inhibition of phloem transport. J Exp Bot 44(9):1491–1496.  https://doi.org/10.1093/jxb/44.9.1491CrossRefGoogle Scholar
  39. 39.
    Pickard WF, Minchin PEH (1990) The transient inhibition of phloem translocation in Phaseolus vulgaris by abrupt temperature drops, vibration, and electric shock. J Exp Bot 41:1361–1369CrossRefGoogle Scholar
  40. 40.
    Strulab D, Santin G, Lazaro D, Breton V, Morel C (2003) GATE (geant4 application for tomographic emission): a PET/SPECT general-purpose simulation platform. Nuclear Phys B Proc Suppl 125:75–79.  https://doi.org/10.1016/S0920-5632(03)90969-8CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.New Zealand Institute for Plant and Food ResearchMotueka Research CentreMotuekaNew Zealand
  2. 2.New Zealand Institute for Plant and Food ResearchMotueka Research CentreMotuekaNew Zealand

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