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Phloem pp 163-176 | Cite as

Detecting Rapid Changes in Carbon Transport and Partitioning with Carbon-11 (11C)

  • Benjamin A. BabstEmail author
  • Richard Ferrieri
  • Michael Schueller
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2014)

Abstract

Noninvasive techniques to measure phloem transport of carbon will be crucial to efforts to engineer improved crop yields, which are highly dependent on carbon partitioning. Phloem, which is buried in the interior of the plant, is highly sensitive to tissue damage. Here we describe nondestructive methods using carbon-11, fed to leaves as 11CO2, as a tracer to track export of recently fixed carbon from leaves, transport speed through the phloem, and distribution or partitioning throughout the plant.

Key words

Photoassimilate transport Phloem Carbon-11 Whole-plant 11C partitioning Carbon allocation 

Notes

Acknowledgment

Preparation of this manuscript was supported in part by the USDA National Institute of Food and Agriculture, McIntire Stennis project 1009319.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Benjamin A. Babst
    • 1
    • 2
    Email author
  • Richard Ferrieri
    • 3
  • Michael Schueller
    • 4
  1. 1.Arkansas Forest Resources CenterMonticelloUSA
  2. 2.University of Arkansas at Monticello, College of Forestry, Agriculture, and Natural ResourcesMonticelloUSA
  3. 3.Missouri Research Reactor Center and Department of ChemistryUniversity of MissouriColumbiaUSA
  4. 4.Department of ChemistryUniversity of Missouri, Missouri Research Reactor CenterColumbiaUSA

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