Using 13C to Quantify Phloem Transport on Tall Plants in the Field
The difference in time lags between a labeling pulse of 13CO2 of the foliage and the appearance of labeled C in the respiration at different locations along the stem of a tall plant is used to estimate at which velocities the isotope tracer, i.e., the labeled carbohydrates, are transported in the phloem sap. Here we describe a method for pulse-labeling tall plants in the field and subsequently tracing 13C in the respiratory efflux of CO2.
Key wordsCarbon transfer Carbon allocation Laser absorption spectroscopy Phloem Pulse labeling Stable carbon isotope Stem CO2 efflux
We thank the many colleagues from Brazil, France, Japan, and Thailand who contributed to the development and improvement of this method.
- 3.Plain C, Gérant D, Maillard P et al (2009) Tracing of recently assimilated carbon in respiration at high temporal resolution in the field with a tuneable diode laser absorption spectrometer after in situ 13CO2 pulse labelling of 20-year-old beech trees. Tree Physiol 29:1433–1447CrossRefGoogle Scholar