Proteomic Response of Host Plants to Citrus tristeza virus

  • Milena Santos Dória
  • Carlos Priminho Pirovani
Part of the Methods in Molecular Biology book series (MIMB, volume 2015)


Proteomics is an excellent technique for detecting proteins involved in plant responses infected with the Citrus tristeza virus. Here we describe the process to extract proteins to obtain two-dimensional gels with a large number of spots, well distributed, and with good quality. The extraction process that associates successive washes with ultrasonication steps proved to be efficient for the detection of proteins. With this technique it was possible to obtain clear gels which were detected in about 600 spots distributed throughout the gel without the presence of drag.

Key words

Proteins Extraction Virus 2D PAGE 



This work was funded by the Conselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq), process N° 304536/2015-6.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Milena Santos Dória
    • 1
  • Carlos Priminho Pirovani
    • 1
  1. 1.Centro de Biotecnologia e GenéticaUniversidade Estadual de Santa CruzIlhéusBrazil

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