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An Animal Model of Alcohol Binge Drinking: Chronic-Intermittent Ethanol Administration in Rodents

  • Santiago MonleónEmail author
  • Aránzazu Duque
  • Patricia Mesa-Gresa
  • Rosa Redolat
  • Concepción Vinader-Caerols
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2011)

Abstract

Binge drinking (BD), characterized by intermittent consumption of large quantities of alcohol in short periods of time, is the main alcohol consumption pattern in adolescents and young adults. BD has serious biomedical consequences, and it is a prominent risk factor for later development of alcohol use disorders. Rodent models offer exceptional power to study these negative consequences of BD. This chapter focuses on one of these BD models: the chronic-intermittent ethanol administration (CIEA) paradigm. Essentially, CIEA consists of the administration in rats or mice of i.p. injections of ethanol (doses: 3–4 g/kg) for several consecutive days each week, in alternation with several days without injections, during several weeks. Due to our interest in the neurobehavioral effects of BD, a combination of the CIEA model with a battery of behavioral tests is described, with emphasis on the effects of alcohol BD on different kinds of memory. The CIEA model, in combination with behavioral tasks, seems to be a useful tool for studying the neurobehavioral effects of BD as well as for developing potential prevention and treatment strategies.

Key words

Animal model Alcohol Binge drinking Chronic-intermittent Ethanol Rats Mice 

Notes

Acknowledgments

This work was supported in part by PROMETEO-II/2015/020 from “Generalitat Valenciana” and UV-INV_AE18-779336 from “Universitat de València”, Spain.

References

  1. 1.
    Observatorio Español sobre Drogas (OED) (2016) Encuesta Escolar sobre Uso de Drogas en Estudiantes de Enseñanzas Secundarias (ESTUDES). Ministerio de Sanidad, Servicios Sociales e Igualdad, Madrid, pp 2014–2015Google Scholar
  2. 2.
    National Institute on Alcohol and Alcoholism (2004) NIAA council approves definition of binge drinking. Department of Health and Human Services. NIAA Newsletter 3:3Google Scholar
  3. 3.
    Courtney KE, Polich J (2009) Binge drinking in young adults: data, definitions, and determinants. Psychol Bull 135:142–156CrossRefGoogle Scholar
  4. 4.
    Crabbe JC, Harris RA, Koob GF (2011) Preclinical studies of alcohol binge drinking. Ann N Y Acad Sci 1216:24–40CrossRefGoogle Scholar
  5. 5.
    Kuntsche E, Kuntsche S, Thrul J, Gmel G (2017) Binge drinking: health impact, prevalence, correlates and interventions. Psychol Health 32:976–1017CrossRefGoogle Scholar
  6. 6.
    Fritz BM, Boehm SL 2nd. (2016) Rodent models and mechanisms of voluntary binge-like ethanol consumption: examples, opportunities, and strategies for preclinical research. Prog Neuro-Psychopharmacol Biol Psychiatry 65:297–308CrossRefGoogle Scholar
  7. 7.
    Sprow GM, Thiele TE (2012) The neurobiology of binge-like ethanol drinking: evidence from rodent models. Physiol Behav 106:325–331CrossRefGoogle Scholar
  8. 8.
    Maldonado-Devincci AM, Badanich KA, Kirstein CL (2010) Alcohol during adolescence selectively alters immediate and long-term behavior and neurochemistry. Alcohol 44:57–66CrossRefGoogle Scholar
  9. 9.
    Sanchez-Roige S, Peña-Oliver Y, Ripley TL, Stephens DN (2014) Repeated ethanol exposure during early and late adolescence: double dissociation of effects on waiting and choice impulsivity. Alcohol Clin Exp Res 38:2579–2589CrossRefGoogle Scholar
  10. 10.
    Lacaille H, Duterte-Boucher D, Liot D, Vaudry H, Naasila M, Vaudry D (2015) Comparison of the deleterious effects of binge drinking-like alcohol exposure in adolescent and adult mice. J Neurochem 132:629–641CrossRefGoogle Scholar
  11. 11.
    Broadwater M, Varlinskaya EI, Spear LP (2011) Chronic intermittent ethanol exposure in early adolescent and adult male rats: effects on tolerance, social behavior, and ethanol intake. Alcohol Clin Exp Res 35:1392–1403PubMedPubMedCentralGoogle Scholar
  12. 12.
    Nogales F, Rua RM, Ojeda ML, Murillo ML, Carreras O (2014) Oral or intraperitoneal binge drinking and oxidative balance in adolescent rats. Chem Res Toxicol 27:1926–1933CrossRefGoogle Scholar
  13. 13.
    Sánchez P, Castro B, Torres JM, Ortega E (2014) Effects of different ethanol-administration regimes on mRNA and protein levels of steroid 5α-reductase isozymes in prefrontal cortex of adolescent male rats. Psychopharmacology 231:3273–3280CrossRefGoogle Scholar
  14. 14.
    Gold PE (1986) The use of avoidance training in studies of modulation of memory storage. Behav Neural Biol 46:87–98CrossRefGoogle Scholar
  15. 15.
    Parra A, Rama E, Vinader-Caerols C, Monleón S (2013) Inhibitory avoidance in CD1 mice: sex matters, as does the supplier. Behav Process 100:36–39CrossRefGoogle Scholar
  16. 16.
    Markwiese BJ, Acheson SK, Levin ED, Wilson WA, Swartzwelder HS (1998) Differential effects of ethanol on memory in adolescent and adult rats. Alcohol Clin Exp Res 22:416–421CrossRefGoogle Scholar
  17. 17.
    Bell RL, Rodd ZA, Engleman EA, Toalston JE, McBride WJ (2014) Scheduled access alcohol drinking by alcohol-preferring (P) and high-alcohol-drinking (HAD) rats: modeling adolescent and adult binge-like drinking. Alcohol 48:225–234CrossRefGoogle Scholar
  18. 18.
    Dutta S, Sengupta P (2016) Men and mice: relating their ages. Life Sci 152:244–248CrossRefGoogle Scholar
  19. 19.
    Alfonso-Loeches S, Pascual M, Guerri C (2013) Gender differences in alcohol-induced neurotoxicity and brain damage. Toxicology 311:27–34CrossRefGoogle Scholar
  20. 20.
    Vinader-Caerols C, Talk A, Montañés A, Duque A, Monleón S (2017) Differential effects of alcohol on memory performance in adolescent men and women with a binge drinking history. Alcohol Alcohol 52:610–616CrossRefGoogle Scholar
  21. 21.
    Knapp DJ, Breese GR (2012) Models of chronic alcohol exposure and dependence. In: Kobeissy F (ed) Psychiatric disorders, Methods in molecular biology (methods and protocols), vol 829. Humana Press, New York, pp 205–230CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Santiago Monleón
    • 1
    Email author
  • Aránzazu Duque
    • 2
  • Patricia Mesa-Gresa
    • 1
  • Rosa Redolat
    • 1
  • Concepción Vinader-Caerols
    • 1
  1. 1.Department of PsychobiologyUniversity of ValenciaValenciaSpain
  2. 2.Universidad Internacional de ValenciaValenciaSpain

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