Currently, gelatin nanoparticles are gaining more grounds in drug and gene delivery throughout all the available several routes of administration. Yet, the homogenous and less disperse preparation of this type of nanoparticles is still a challenging task due to the variation of the gelatin quality according to its source and due to its variable molecular weight. Accordingly, several methods were proposed from which the double-desolvation method has been proven to yield optimum results regarding particle size and homogeneity. Thereby, we describe in this chapter a detailed procedure of this method. We also introduce our protocols of the cationization of this kind of nanoparticles as it is extensively needed in case of loading genetic materials or proteins. Additionally, FITC labeling of gelatin nanoparticles that is usually utilized for purposes of imaging or bio-distribution studies is also introduced step by step.
Key wordsGelatin Nanoparticles Desolvation Cationization FITC Labeling Drug delivery Gene
The authors would like to thank Dr. Reza Nejadnik, Leiden/Amsterdam Centre for Drug Research, for introducing us to several important points in the preparation of gelatin nanoparticles.
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