Protein Microarrays and Liposome: A Method for Studying Lipid–Protein Interactions

  • Samuel Herianto
  • Chien-Sheng Chen
  • Heng ZhuEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2003)


Interactions between specific lipids and proteins can provide important information regarding the functions of proteins and lipids. One of the novel and powerful methods to identify lipid–protein interactions is using protein microarrays and liposomes. Liposomes are spherical vesicles that are surrounded by phospholipid bilayers in which the lipid of interest can be incorporated. Thus, liposomes can be used to detect lipid–protein interactions and to analyze interactions between thousands of proteins and a small number of lipids with a single experiment. This chapter presents the methods and procedures for using protein microarray assays and liposome fabrication to analyze protein–lipid interactions. Up-to-date research reports are also reviewed briefly.

Key words

Protein microarrays Protein–lipid interactions High throughput Liposomes 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.High Throughput Biosensing Laboratory, Department of Food Safety/Hygiene and Risk Management, College of MedicineNational Cheng Kung UniversityTainan CityTaiwan
  2. 2.Department of Pharmacology and Molecular Sciences/High-Throughput Biology Center, School of MedicineJohns Hopkins UniversityBaltimoreUSA

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