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Protein Microarrays and Liposome: A Method for Studying Lipid–Protein Interactions

  • Samuel Herianto
  • Chien-Sheng Chen
  • Heng ZhuEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2003)

Abstract

Interactions between specific lipids and proteins can provide important information regarding the functions of proteins and lipids. One of the novel and powerful methods to identify lipid–protein interactions is using protein microarrays and liposomes. Liposomes are spherical vesicles that are surrounded by phospholipid bilayers in which the lipid of interest can be incorporated. Thus, liposomes can be used to detect lipid–protein interactions and to analyze interactions between thousands of proteins and a small number of lipids with a single experiment. This chapter presents the methods and procedures for using protein microarray assays and liposome fabrication to analyze protein–lipid interactions. Up-to-date research reports are also reviewed briefly.

Key words

Protein microarrays Protein–lipid interactions High throughput Liposomes 

References

  1. 1.
    Chen CS, Zhu H (2006) Protein microarrays. Biotechniques 40(4):423.  https://doi.org/10.2144/06404te01CrossRefPubMedGoogle Scholar
  2. 2.
    Zhu H, Bilgin M, Bangham R, Hall D, Casamayor A, Bertone P, Lan N, Jansen R, Bidlingmaier S, Houfek T, Mitchell T, Miller P, Dean RA, Gerstein M, Snyder M (2001) Global analysis of protein activities using proteome chips. Science 293(5537):2101–2105.  https://doi.org/10.1126/science.1062191CrossRefPubMedGoogle Scholar
  3. 3.
    Lu KY, Tao SC, Yang TC, Ho YH, Lee CH, Lin CC, Juan HF, Huang HC, Yang CY, Chen MS, Lin YY, Lu JY, Zhu H, Chen CS (2012) Profiling lipid-protein interactions using nonquenched fluorescent liposomal nanovesicles and proteome microarrays. Mol Cell Proteomics 11(11):1177–1190.  https://doi.org/10.1074/mcp.M112.017426CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Lu KY, Chen CS, Neiswinger J, Zhu H (2016) Characterization of lipid-protein interactions using nonquenched fluorescent liposomal nanovesicles and yeast proteome microarrays. Cold Spring Harb Protoc 2016(10):pdb.prot087981.  https://doi.org/10.1101/pdb.prot087981CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.High Throughput Biosensing Laboratory, Department of Food Safety/Hygiene and Risk Management, College of MedicineNational Cheng Kung UniversityTainan CityTaiwan
  2. 2.Department of Pharmacology and Molecular Sciences/High-Throughput Biology Center, School of MedicineJohns Hopkins UniversityBaltimoreUSA

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