Use of Human Monocyte-Derived Macrophages to Study Neisseria gonorrhoeae Infection
Macrophages are critical cells in the innate immune response to microorganisms sensed in the tissues. During infections, the interaction between pathogens and macrophages leads to a macrophage response that includes cytokine production, antigen processing and presentation in the context of MHC molecules, expression of T cell costimulatory molecules and recruitment of innate defense effectors, which results in clearance of infection. However, Neisseria gonorrhoeae can suppress the protective immune response at this level, avoiding its detection and elimination. Studies addressed to develop the interactions between macrophages and Neisseria gonorrhoeae allow us to find potential targets to be exploited with vaccines and therapeutic drugs. In this chapter, we describe protocols to generate human monocyte-derived macrophages and assess their response to infection with Neisseria gonorrhoeae.
Key wordsNeisseria gonorrhoeae Monocyte-derived macrophages Phagocytosis Cytokines T cell proliferation
This work was supported by FONDECYT regular 1180666 (J.G., P.I.R., and A.E.). PCI-CONICYT ‘Apoyo a la Formación de Redes Internacionales Para Investigadores en Etapa Inicial’ REDI170370 (P.I.R., A.E.).
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