Use of Human Monocyte-Derived Macrophages to Study Neisseria gonorrhoeae Infection

  • Jimena Gatica
  • Paula I. Rodas
  • Alejandro Escobar
Part of the Methods in Molecular Biology book series (MIMB, volume 1997)


Macrophages are critical cells in the innate immune response to microorganisms sensed in the tissues. During infections, the interaction between pathogens and macrophages leads to a macrophage response that includes cytokine production, antigen processing and presentation in the context of MHC molecules, expression of T cell costimulatory molecules and recruitment of innate defense effectors, which results in clearance of infection. However, Neisseria gonorrhoeae can suppress the protective immune response at this level, avoiding its detection and elimination. Studies addressed to develop the interactions between macrophages and Neisseria gonorrhoeae allow us to find potential targets to be exploited with vaccines and therapeutic drugs. In this chapter, we describe protocols to generate human monocyte-derived macrophages and assess their response to infection with Neisseria gonorrhoeae.

Key words

Neisseria gonorrhoeae Monocyte-derived macrophages Phagocytosis Cytokines T cell proliferation 



This work was supported by FONDECYT regular 1180666 (J.G., P.I.R., and A.E.). PCI-CONICYT ‘Apoyo a la Formación de Redes Internacionales Para Investigadores en Etapa Inicial’ REDI170370 (P.I.R., A.E.).


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Jimena Gatica
    • 1
  • Paula I. Rodas
    • 2
  • Alejandro Escobar
    • 3
  1. 1.Instituto de Investigación en Ciencias Odontológicas, Facultad de OdontologíaUniversidad de ChileSantiagoChile
  2. 2.Laboratory of Medical Microbiology and Pathogenesis, Faculty of MedicineUniversidad Andres BelloConcepciónChile
  3. 3.Laboratorio Biología celular y molecular, Instituto de Ciencias Odontológicas, Facultad de OdontologíaUniversidad de ChileSantiagoChile

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