Advertisement

Producing Oleaginous Organisms Using Food Waste: Challenges and Outcomes

  • Singaram Jayanthi
  • Arun Kumar Thalla
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1995)

Abstract

With organic or food waste being one of the main constituents of the total urban waste generated, it not only makes it essential to seek means for its safe disposal but at the same time reiterates the huge potential that lies with the proper utilization of such a widely available resource. Oleaginous microbes that are effective in producing or storing oil would use food waste rich in carbohydrates, lipids, and proteins, and this oil in turn could be an alternative feedstock for the production of biofuels. However, there are few challenges in the process. The various challenges in this process and methods to address them are discussed in the present chapter.

Key words

Food waste Oleaginous Lipids Biodiesel 

References

  1. 1.
    USEPA Sustainable Management of Food, https://www.epa.gov/sustainable-management-food/food-recovery-hierarchy. Accessed 24 Apr 2018
  2. 2.
    Thevenieau F, Nicaud JM (2013) Microorganisms as sources of oils. OCL 20(6):D603CrossRefGoogle Scholar
  3. 3.
    Lin J, Shen H, Tan H, Zhao X, Wu S, Hu C, Zhao ZK (2011) Lipid production by Lipomyces starkeyi cells in glucose solution without auxiliary nutrients. J Biotechnol 152(4):184–188CrossRefGoogle Scholar
  4. 4.
    Wu S, Zhao X, Shen H, Wang Q, Zhao ZK (2011) Microbial lipid production by Rhodosporidium toruloides under sulfate-limited conditions. Bioresour Technol 102(2):1803–1807CrossRefGoogle Scholar
  5. 5.
    Wiebe MG, Koivuranta K, Penttilä M, Ruohonen L (2012) Lipid production in batch and fed-batch cultures of Rhodosporidium toruloides from 5 and 6 carbon carbohydrates. BMC Biotechnol 12(1):26CrossRefGoogle Scholar
  6. 6.
    Kumar SV, Kumutha K, Krishnan PS, Gopal H (2010) Influence of nitrogen on lipid and biomass production by oleaginous yeast cultures. Asian J Biol Sci 5(1):87–91Google Scholar
  7. 7.
    Scott C, Hilton ME, Coppin CW, Russell RJ, Oakeshott JG, Sutherland TD (2007) A global response to sulfur starvation in Pseudomonas putida and its relationship to the expression of low-sulfur-content proteins. FEMS Microbiol Lett 267(2):184–193CrossRefGoogle Scholar
  8. 8.
    Spencer JFT, Spencer DM, de Figueroa LIC (1997) Yeasts as living objects: yeast nutrition. In: Yeasts in natural and artificial habitats. Springer, Berlin, Heidelberg, pp 68–79CrossRefGoogle Scholar
  9. 9.
    Rattray JB, Schibeci A, Kidby DK (1975) Lipids of yeasts. Bacteriol Rev 39(3):197PubMedPubMedCentralGoogle Scholar
  10. 10.
    Haskell BE, Snell EE (1965) Effect of vitamin B6 deficiency on the composition of yeast lipids. Arch Biochem Biophys 112(3):494–505CrossRefGoogle Scholar
  11. 11.
    Angerbauer C, Siebenhofer M, Mittelbach M, Guebitz GM (2008) Conversion of sewage sludge into lipids by Lipomyces starkeyi for biodiesel production. Bioresour Technol 99(8):3051–3056CrossRefGoogle Scholar
  12. 12.
    Brown DE, Hasan M, Lepe-Casillas M, Thornton AJ (1990) Effect of temperature and pH on lipid accumulation by Trichoderma reesei. Appl Microbiol Biotechnol 34(3):335–339CrossRefGoogle Scholar
  13. 13.
    Babij T, Moss FJ, Ralph BJ (1969) Effects of oxygen and glucose levels on lipid composition of yeast Candida utilis grown in continuous culture. Biotechnol Bioeng 11(4):593–603CrossRefGoogle Scholar
  14. 14.
    Valero E, Millán C, Ortega J (2001) Influence of oxygen addition during growth phase on the biosynthesis of lipids in Saccharomyces cerevisiae (M330-9) in enological fermentations. J Biosci Bioeng 92(1):33–38CrossRefGoogle Scholar
  15. 15.
    Beopoulos A, Mrozova Z, Thevenieau F, Le Dall MT, Hapala I, Papanikolaou S, Nicaud JM (2008) Control of lipid accumulation in the yeast Yarrowia lipolytica. Appl Environ Microbiol 74(24):7779–7789CrossRefGoogle Scholar
  16. 16.
    Analysis of biomolecules. http://nptel.ac.in/courses/102103045/module5/lec28/4.html. Accessed Sep 2017
  17. 17.
    Lichtenberg D, Ahyayauch H, Goñi FM (2013) The mechanism of detergent solubilization of lipid bilayers. Biophys J 105(2):289–299CrossRefGoogle Scholar
  18. 18.
    Cell Disruption. http://upendrats.blogspot.in/2012/09/cell-disruption.html. Accessed 24 Apr 2018
  19. 19.
    Folch J, Lees M, Sloane Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226(1):497–509PubMedPubMedCentralGoogle Scholar
  20. 20.
    Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37(8):911–917CrossRefGoogle Scholar
  21. 21.
    Markham JE, Li J, Cahoon EB, Jaworski JG (2006) Separation and identification of major plant sphingolipid classes from leaves. J Biol Chem 281(32):22684–22694CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Singaram Jayanthi
    • 1
  • Arun Kumar Thalla
    • 2
  1. 1.Government College of TechnologyCoimbatoreIndia
  2. 2.Department of Civil EngineeringNational Institute of Technology KarnatakaMangaloreIndia

Personalised recommendations