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Mouse Embryo Culture for the Study of Neural Crest Cells

  • William A. Muñoz
  • Paul A. TrainorEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1976)

Abstract

Mammalian development occurs in utero, which makes it difficult to study the diverse morphogenetic events of neural crest cell development in vivo. Analyses of fixed samples in conjunction with histological methods to evaluate the spatiotemporal roles of specific genes of interest only provide snapshots of mammalian neural crest cell development. This chapter describes methods for isolating and culturing mouse embryos and their organs in vitro, outside the mother, to facilitate real-time imaging and functional analyses of the dynamics of neural crest cell development.

Key words

Mouse embryo Whole embryo culture Live imaging Development Gene transfer Neural crest cell Roller culture Electroporation Lipofection 

Notes

Acknowledgments

Work in the Trainor laboratory is supported by the Stowers Institute for Medical Research and the National Institute of Dental and Craniofacial Research (DE016082). WAM is supported by the American Association of Anatomists Postdoctoral Fellowship.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Stowers Institute for Medical ResearchKansas CityUSA
  2. 2.Department of Anatomy and Cell BiologyUniversity of Kansas Medical CenterKansas CityUSA

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