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Live Imaging of Neural Crest and Pigment Cells and Transient Transgenic Manipulation of Gene Activity

  • Reyna Deeya Ballim
  • Yusuke Nagao
  • Robert Neil KelshEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1976)

Abstract

Neural crest cells are a highly multipotent and migratory cell type that are important for adult pigment pattern formation, cellular homeostasis, and regeneration. The optical transparency and accessibility of fish embryos makes them particularly well-suited to high-resolution analysis of neural crest development. However, the dispersive nature of these cells adds to the challenge of their study. We describe key protocols for the analysis of neural crest development in zebrafish and medaka, including live imaging of neural crest cells and differentiating pigment cells and transient transgenesis assays that can be used to manipulate neural crest development.

Key words

Neural crest Pigment cells Zebrafish Medaka Live imaging Melanocyte Xanthophore Leucophore Iridophore Transient transgenesis Tol2 

Notes

Acknowledgments

This work was supported by BBSRC grant BB/L00769X/1 (R.N.K.), Marie Sklodowska-Curie grant No. 661616 (R.D.B.), and Japanese Society for the Promotion of Research Program for Advancing Strategic International Networks to Accelerate the Circulation of Talented Researchers S2602 (Y.N.) and Overseas Research Fellowships No. 598 (Y.N.).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Reyna Deeya Ballim
    • 1
  • Yusuke Nagao
    • 1
  • Robert Neil Kelsh
    • 1
    Email author
  1. 1.Department of Biology and BiochemistryUniversity of BathBathUK

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