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Leishmania pp 225-235 | Cite as

DiCre-Based Inducible Gene Expression

  • Jeziel D. DamascenoEmail author
  • Luiz R. O. Tosi
  • Renato E. R. S. Santos
  • Jeremy C. Mottram
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1971)

Abstract

Induction of gene expression is a valuable approach for functional studies since it allows for the assessment of phenotypes without the need for clonal selection. Inducible expression can find a wide range of applications, from the study of essential genes to the characterization of overexpression of genes of interest. Here, we describe a detailed protocol for the use of the DiCre-based inducible gene expression system in Leishmania parasites. This is a tightly regulated induction system that allows for time- and dose-controlled expression of gene products, as rapidly as within 12 h.

Key words

Dimerizable cre recombinase DiCre Inducible gene expression Conditional genome engineering Leishmania Trypanosomatids 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Jeziel D. Damasceno
    • 1
    Email author
  • Luiz R. O. Tosi
    • 2
  • Renato E. R. S. Santos
    • 2
  • Jeremy C. Mottram
    • 3
  1. 1.The Wellcome Trust Centre for Molecular Parasitology, Institute of Infection, Immunity and InflammationUniversity of GlasgowGlasgowUK
  2. 2.Department of Cell and Molecular Biology, Ribeirão Preto Medical SchoolUniversity of São PauloRibeirão PretoBrazil
  3. 3.Department of Biology, York Biomedical Research InstituteUniversity of YorkYorkUK

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