The Detroit 562 Pharyngeal Immortalized Cell Line Model for the Assessment of Infectivity of Pathogenic Neisseria sp.
Neisseria meningitidis and Neisseria gonorrhoeae are obligate pathogens of the human host. Due to their adaptation to the human host, many factors required for infection are specialized for the human host to the point that natural infection processes are difficult to replicate in animal models. Immortalized human cell lines have been used to identify the host factors necessary for successful colonization of human mucosal surfaces. One such model is the Detroit 562 pharyngeal immortalized cell monolayer model which is used to measure the rate of attachment to and invasion of N. meningitidis and N. gonorrhoeae into epithelial cells. The methodology of this assay, as well as the maintenance of Detroit 562 cells necessary for the experiment, will be described.
Key wordsDetroit 562 Pharyngeal Meningococcal Gonococcal Host receptors
This work is supported by the Australian Defence Material Transfer Center (Project Agreement 10.44) and North Atlantic Treaty Organization (SfP-984235) awarded to MST. EK is supported by a Research Training Program (RTP) PhD scholarship from Murdoch University. CK is supported by the Amanda Young Foundation, a not-for-profit organisation, for improved community awareness of meningococcal disease.
- 1.Islam EA, Gray-Owen SD (2014) Modelling infection by the pathogenic Neisseria. In: Davies JK, Kahler CM (eds) Pathogenic Neisseria: Genomics, Molecular Biology and Disease Prevention. Caister Academic Press, NorfolkGoogle Scholar
- 6.Mubaiwa TD, Semchenko EA, Hartley-Tassell LE, Day CJ, Jennings MP, Seib KL (2017) The sweet side of the pathogenic Neisseria: the role of glycan interactions in colonisation and disease. Pathog Dis 75. https://doi.org/10.1093/femspd/ftx063
- 7.Weyand NJ (2017) Neisseria models of infection and persistence in the upper respiratory tract. Pathog Dis 75. https://doi.org/10.1093/femspd/ftx031
- 9.Bartley SN, Tzeng YL, Heel K, Lee CW, Mowlaboccus S, Seemann T, Lu W, Lin YH, Ryan CS, Peacock C, Stephens DS, Davies JK, Kahler CM (2013) Attachment and invasion of Neisseria meningitidis to host cells is related to surface hydrophobicity, bacterial cell size and capsule. PLoS One 8:e55798CrossRefGoogle Scholar