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Modeling Neisseria meningitidis Infection in Mice: Methods and Logistical Considerations for Nasal Colonization and Invasive Disease

  • Kay O. Johswich
  • Scott D. Gray-OwenEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1969)

Abstract

The single greatest barrier to studying the lifestyle of Neisseria meningitidis stems from its exquisite adaptation to life in humans, a specialization which prevents it from infecting other animals. This barrier to modeling meningococcal infection has been overcome by the provision of factors that allow the meningococci to overcome one or more aspects of host restriction, including the use of mice expressing receptors that allow mucosal colonization and/or the inclusion of serum factors that facilitate meningococcal replication during disseminated meningococcal disease. Here we discuss these advances, consider variables that influence the outcome of infection, and detail the technical requirements to establish robust and reproducible nasal colonization or sepsis. Once established, these models can then be used to study the meningococcal lifestyle and the immune response during infection, and to facilitate development of novel drug or vaccine-based approaches to intervene in meningococcal carriage and disease.

Key words

Mouse model Intraperitoneal infection Sepsis Nasal infection Mucosal colonization Transgenic mice 

References

  1. 1.
    Schmitz JES, Stratton CW (2015) Neisseria meningitidis. In: Tang Y-W, Sussman M, Liu D, Poxton I, Schwartzman J (eds) Molecular medical microbiology, vol 3, 2nd edn. Academic Press, Waltham, MA, pp 1729–1750Google Scholar
  2. 2.
    Holbein BE, Jericho KWF, Likes GC (1979) Neisseria meningitidis infection in mice: influence of iron, variations in virulence among strains, and pathology. Infect Immun 24:545–551Google Scholar
  3. 3.
    Holbein BE (1981) Enhancement of Neisseria meningitidis infection in mice by addition of iron bound to transferrin. Infect Immun 34:120–125Google Scholar
  4. 4.
    Schryvers AB, Morris LJ (1988) Identification and characterization of the transferrin receptor from Neisseria meningitidis. Mol Microbiol 2:281–288Google Scholar
  5. 5.
    Morgenthau A, Pogoutse A, Adamiak P, Moraes TF, Schryvers AB (2013) Bacterial receptors for host transferrin and lactoferrin: molecular mechanisms and role in host-microbe interactions. Future Microbiol 8:1575–1585CrossRefGoogle Scholar
  6. 6.
    Szatanik M, Hong E, Ruckly C, Ledroit M, Giorgini D, Jopek K, Nicola MA, Deghmane AE, Taha MK (2011) Experimental meningococcal sepsis in congenic transgenic mice expressing human transferrin. PLoS One 6:e22210CrossRefGoogle Scholar
  7. 7.
    Zarantonelli ML, Szatanik M, Giorgini D, Hong E, Huerre M, Guillou F, Alonso JM, Taha MK (2007) Transgenic mice expressing human transferrin as a model for meningococcal infection. Infect Immun 75:5609–5614CrossRefGoogle Scholar
  8. 8.
    Lewis LA, Ram S (2014) Meningococcal disease and the complement system. Virulence 5:98–126CrossRefGoogle Scholar
  9. 9.
    Vu DM, Shaughnessy J, Lewis LA, Ram S, Rice PA, Granoff DM (2012) Enhanced bacteremia in human factor H transgenic rats infected by Neisseria meningitidis. Infect Immun 80:643–650Google Scholar
  10. 10.
    Johswich KO, McCaw SE, Strobel L, Frosch M, Gray-Owen SD (2015) Sterilizing immunity elicited by Neisseria meningitidis carriage shows broader protection than predicted by serum antibody cross-reactivity in CEACAM1-humanized mice. Infect Immun 83:354–363CrossRefGoogle Scholar
  11. 11.
    Johswich KO, McCaw SE, Islam E, Sintsova A, Gu A, Shively JE, Gray-Owen SD (2013) In vivo adaptation and persistence of Neisseria meningitidis within the nasopharyngeal mucosa. PLoS Pathog 9:e1003509Google Scholar
  12. 12.
    Crockett ET, Spielman W, Dowlatshahi S, He J (2006) Sex differences in inflammatory cytokine production in hepatic ischemia-reperfusion. J Inflamm 3:16CrossRefGoogle Scholar
  13. 13.
    Boivin GP, Hickman DL, Creamer-Hente MA, Pritchett-Corning KR, Bratcher NA (2017) Review of CO(2) as a euthanasia agent for laboratory rats and mice. J Am Assoc Lab Anim Sci 56:491–499PubMedPubMedCentralGoogle Scholar
  14. 14.
    Brener D, DeVoe IW, Holbein BE (1981) Increased virulence of Neisseria meningitidis after in vitro iron-limited growth at low pH. Infect Immun 33:59–66Google Scholar
  15. 15.
    Johswich K (2017) Innate immune recognition and inflammation in Neisseria meningitidis infection. Pathog Dis 75.  https://doi.org/10.1093/femspd/ftx022
  16. 16.
    Alonso JM, Guiyoule A, Zarantonelli ML, Ramisse F, Pires R, Antignac A, Deghmane AE, Huerre M, van der Werf S, Taha MK (2003) A model of meningococcal bacteremia after respiratory superinfection in influenza A virus-infected mice. FEMS Microbiol Lett 222:99–106CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute for Hygiene and MicrobiologyUniversity of WürzburgWürzburgGermany
  2. 2.Department of Molecular GeneticsUniversity of TorontoTorontoCanada

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