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High-Resolution and Super-Resolution Immunofluorescent Microscopy Ex Vivo to Study Pneumococcal Interactions with the Host

  • Federico IovinoEmail author
  • Birgitta Henriques-Normark
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1968)

Abstract

In vivo imaging, meaning imaging tissues in living animals, is still a developing technique. However, microscopy imaging ex vivo remains a very important tool that allows for visualization of biological and pathological processes occurring in vivo. As described in Chap. 5, imaging of animal and human tissue postmortem can be performed at high resolution. Recently, imaging of human tissues infected with pneumococci using an even higher resolution, the so-called super-resolution with STED, has been reported.

Key words

High-Resolution microscopy Super-Resolution Mouse tissue Human tissue Immunofluorescence 

References

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Federico Iovino
    • 1
    • 2
    Email author
  • Birgitta Henriques-Normark
    • 1
    • 2
    • 3
  1. 1.Department of Microbiology, Tumor and Cell BiologyKarolinska Institutet, BioclinicumStockholmSweden
  2. 2.Department of Clinical MicrobiologyKarolinska University HospitalStockholmSweden
  3. 3.Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Lee Kong Chian School of Medicine (LKC)Nanyang Technological University (NTU)SingaporeSingapore

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