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Immunofluorescent Staining and High-Resolution Microscopy to Study the Pneumococcal Cell

  • Federico IovinoEmail author
  • Birgitta Henriques-Normark
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1968)

Abstract

Immunofluorescent staining using antibodies to detect specific proteins allows for visualization of proteins of interest in a biological sample. In recent years, there have been important advances in the microscopy equipment used for imaging, and we can now perform so-called high-resolution microscopy. Through high-resolution microscopy we can not only study biological processes but also visualize them.

Key words

High-resolution microscopy Pneumococcal cell Immunofluorescence 

References

  1. 1.
    Iovino F, Orihuela CJ, Moorlag HE et al (2013) Interactions between blood–borne Streptococcus pneumoniae and the blood–brain barrier preceding meningitis. PLoS One 8:e68408CrossRefGoogle Scholar
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    Iovino F, Hammarlöf DL, Garriss G et al (2016) Pneumococcal meningitis is promoted by single cocci expressing pilus adhesin RrgA. J Clin Invest 126:2821–2826CrossRefGoogle Scholar
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    Iovino F, Molema G, Bijlsma JJ (2014) Platelet endothelial cell adhesion molecule-1, a putative receptor for the adhesion of Streptococcus pneumoniae to the vascular endothelium of the blood–brain barrier. Infect Immun 82:3555–3566CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Federico Iovino
    • 1
    • 2
    Email author
  • Birgitta Henriques-Normark
    • 1
    • 2
    • 3
  1. 1.Department of Microbiology, Tumor and Cell BiologyKarolinska Institutet, BioclinicumStockholmSweden
  2. 2.Department of Clinical MicrobiologyKarolinska University HospitalStockholmSweden
  3. 3.Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Lee Kong Chian School of Medicine (LKC)Nanyang Technological University (NTU)SingaporeSingapore

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