Value of DSC in Characterization and Optimization of Protein Stability

  • Katherine BowersEmail author
  • Natalia MarkovaEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1964)


Differential scanning calorimetry (DSC) is a well-established technique for biomolecular stability studies. The technique is based on forced thermal denaturation of biomolecules in solution. Here we describe the use of DSC for characterization and optimization of stability of two proteins, a protein kinase and a mAb protein.

Key words

Differential scanning calorimetry DSC Protein stability Buffer optimization Multiple stability metrics Pre-formulation 



The Sprint Bioscience AB (Huddinge, Sweden) is acknowledged for providing constructs of protein kinase.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Principal Scientist/Group Leader Analytical and Formulation Development, FUJIFILM Diosynth Biotechnologies U.S.A., Inc.MorrisvilleUSA
  2. 2.MicroCal, Malvern PanalyticalUppsalaSweden

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