Enzyme Kinetics Determined by Single-Injection Isothermal Titration Calorimetry

  • Colette F. QuinnEmail author
  • Lee D. Hansen
Part of the Methods in Molecular Biology book series (MIMB, volume 1964)


This chapter describes how to collect Michaelis-Menten kinetic data on an enzyme-catalyzed reaction with the isothermal titration calorimetry (ITC) and the single-injection method. ITC measures the heat rate which is directly proportional to the reaction rate. The enthalpy change (ΔrH), Km, kcat, and vmax are determined in a single assay that does not require labeling or immobilization.

Key words

Kinetics ITC Michaelis-Menten Enzyme Single-injection method Calorimetry Enthalpy 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Applications LabTA InstrumentsLindonUSA
  2. 2.Department of Chemistry and BiochemistryBrigham Young UniversityProvoUSA

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