Analysis of Isothermal Titration Calorimetry Data for Complex Interactions Using I2CITC

  • Ibrahim Q. Saeed
  • Niklaas J. BuurmaEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1964)


I2CITC allows the analysis of isothermal titration calorimetry (ITC) data for complex coupled equilibria. Here we describe how, using I2CITC, ITC data for systems involving a self-aggregating ligand and a host offering one or two binding sites can be analyzed, how interaction models can be tested, and how confidence intervals for the optimized parameters can be determined.

Key words

Isothermal titration calorimetry ITC Data analysis I2CITC Confidence interval Complex equilibrium systems Thermodynamics 



Users of ICITC and I2CITC, past and present, are thanked for their feedback and questions which have contributed to the improvement of the software over the years.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Chemistry, College of ScienceSalahaddin UniversityErbilIraq
  2. 2.Physical Organic Chemistry Centre, School of ChemistryCardiff UniversityCardiffUK

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