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Characterization of Microtubule-Associated Proteins (MAPs) and Tubulin Interactions by Isothermal Titration Calorimetry (ITC)

  • Philipp O. Tsvetkov
  • Romain La Rocca
  • Soazig Malesinski
  • François DevredEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1964)

Abstract

Microtubules are highly dynamic structures which play a central role in many cellular processes such as cell division, intracellular transport, and migration. Their dynamics is tightly regulated by stabilizing and destabilizing microtubule-associated proteins (MAPs), such as tau and stathmin. Many approaches have been developed to study interactions between tubulin and MAPs. However, isothermal titration calorimetry (ITC) is the only direct thermodynamic method that enables a full thermodynamic characterization of the interaction after a single titration experiment. We provide here the protocols to apply ITC to tubulin interaction with either stathmin or tau, which will help to avoid the common pitfalls in this very powerful and sensitive method.

Key words

Tubulin Microtubule Isothermal titration calorimetry Stathmin Tau 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Philipp O. Tsvetkov
    • 1
  • Romain La Rocca
    • 1
  • Soazig Malesinski
    • 1
  • François Devred
    • 1
    Email author
  1. 1.Aix-Marseille Univ, CNRS, INP, Inst Neurophysiopathol, Fac PharmMarseilleFrance

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