Ancient DNA pp 31-44 | Cite as

Sampling and Extraction of Ancient DNA from Sediments

  • Laura S. Epp
  • Heike H. Zimmermann
  • Kathleen R. Stoof-Leichsenring
Part of the Methods in Molecular Biology book series (MIMB, volume 1963)


Environmental DNA preserved in sediments is rapidly gaining importance as a tool in paleoecology. Sampling procedures for sedimentary ancient DNA (sedaDNA) have to be well planned to ensure clean subsampling of the inside of sediment cores and avoid introducing contamination. Additionally, ancient DNA extraction protocols may need to be optimized for the recovery of DNA from sediments, which may contain inhibitors. Here we describe procedures for subsampling both nonfrozen and frozen sediment cores, and we describe an efficient method for ancient DNA extraction from such samples.

Key words

Sedimentary ancient DNA (sedaDNA) Environmental DNA (eDNA) Sediment cores Permafrost Metagenomics Metabarcoding 



This work was supported by the DFG grant EP98/2-1 to LSE. We thank Inger G. Alsos for exchange of experiences concerning the DNA extraction protocols.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Laura S. Epp
    • 1
    • 2
  • Heike H. Zimmermann
    • 1
  • Kathleen R. Stoof-Leichsenring
    • 1
  1. 1.Alfred Wegener Institute Helmholtz Centre for Polar and Marine ResearchPolar Terrestrial Environmental SystemsPotsdamGermany
  2. 2.Department of BiologyUniversity of KonstanzKonstanzGermany

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