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Ancient DNA pp 25-29 | Cite as

Extraction of Highly Degraded DNA from Ancient Bones and Teeth

  • Jesse Dabney
  • Matthias MeyerEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1963)

Abstract

We provide a DNA extraction protocol optimized for the recovery of highly fragmented molecules preserved within bones and teeth. In this method, the hard tissue matrix is degraded using an EDTA/Proteinase K lysis buffer, and the DNA is purified using spin columns with silica membranes. This method efficiently recovers molecules as short as 35 base-pairs long.

Key words

Ancient DNA DNA extraction Degraded DNA Silica purification Paleogenomics 

References

  1. 1.
    Allentoft ME, Collins M, Harker D, Haile J, Oskam CL, Hale ML et al (2012) The half-life of DNA in bone: measuring decay kinetics in 158 dated fossils. Proc R Soc B 279(1748):4724–4733CrossRefGoogle Scholar
  2. 2.
    Dabney J, Knapp M, Glocke I, Gansauge M-T, Weihmann A, Nickel B et al (2013) Complete mitochondrial genome sequence of a middle Pleistocene cave bear reconstructed from ultrashort DNA fragments. Proc Natl Acad Sci USA 110(39):15758–15763CrossRefGoogle Scholar
  3. 3.
    Meyer M, Fu Q, Aximu-Petri A, Glocke I, Nickel B, Arsuaga J-L et al (2014) A mitochondrial genome sequence of a hominin from Sima de los Huesos. Nature 505(7483):403–406CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Physiology, Anatomy and GeneticsUniversity of OxfordOxfordUK
  2. 2.Department of Evolutionary GeneticsMax Planck Institute for Evolutionary AnthropologyLeipzigGermany

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