Ancient DNA pp 25-29 | Cite as

Extraction of Highly Degraded DNA from Ancient Bones and Teeth

  • Jesse Dabney
  • Matthias MeyerEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1963)


We provide a DNA extraction protocol optimized for the recovery of highly fragmented molecules preserved within bones and teeth. In this method, the hard tissue matrix is degraded using an EDTA/Proteinase K lysis buffer, and the DNA is purified using spin columns with silica membranes. This method efficiently recovers molecules as short as 35 base-pairs long.

Key words

Ancient DNA DNA extraction Degraded DNA Silica purification Paleogenomics 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Physiology, Anatomy and GeneticsUniversity of OxfordOxfordUK
  2. 2.Department of Evolutionary GeneticsMax Planck Institute for Evolutionary AnthropologyLeipzigGermany

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