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Ancient DNA pp 121-128 | Cite as

Hybridization Capture of Ancient DNA Using RNA Baits

  • André E. R. Soares
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1963)

Abstract

The majority of DNA recovered from ancient remains is derived from organisms that colonize the remains post-mortem, such as soil microbes, or from contaminants, such as DNA from living humans. Additionally, some ancient DNA research projects aim to target specific genomic regions, such as mitochondrial genomes or variable single nucleotide polymorphisms (SNPs). To overcome the challenge of targeting specific fragments of DNA from within a complex DNA extract, methods have been developed to enrich ancient DNA extracts for target DNA relative to nontarget DNA. This chapter describes a method for target DNA enrichment that uses hybridization to biotinylated RNA baits to capture and amplify specific ancient DNA fragments from within the pool of extracted fragments.

Key words

Ancient DNA DNA capture RNA bait Hybridization 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • André E. R. Soares
    • 1
  1. 1.Laboratório Nacional de Computação CientíficaPetrópolisBrazil

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