Conjugation of Synthetic Oligosaccharides to Proteins by Squaric Acid Chemistry
Oligosaccharides equipped with amine-containing linkers can be conjugated to carrier proteins using squaric acid chemistry. In a two-step process, a squarate derivative of such oligosaccharide is formed first, which is followed by its reaction with a protein carrier. Monitoring of the conjugation reaction is achieved by SELDI-TOF-MS or MALDI-TOF-MS. This experimentally simple procedure yields desired glycoconjugates in high yields and with reproducible hapten–protein ratios.
Key wordsOligosaccharides Neoglycoconjugates Squaric acid chemistry Conjugation Vaccines
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