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Immunohistochemical Detection of Alpha-Synuclein in Unfixed Human Brain Tissue

  • John B. Sanderson
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1948)

Abstract

In both research and diagnostics, immunohistochemistry is an essential method for assessing pathology in neurodegenerative diseases. Typically, at autopsy, one hemisphere of the brain is formalin fixed for sectioning and histochemical analysis, while the other hemisphere is flash frozen for biochemical studies. While this process ensures the generation of high-quality slides, it is important to assess the pathology of tissue near pieces used for biochemical analyses, since pathology burden can be unevenly distributed throughout the brain (Fig. 1). Here, we describe a method for assessing alpha-synuclein (αSyn) pathology in unfixed human brain tissue using a novel monoclonal antibody.

Key words

Alpha-synuclein Immunohistochemistry Human tissue Frozen Fresh 

Notes

Acknowledgments

The author would like to thank Dr. Mel Feany, Dr. Liqun Wang, and Kit Sin Tuen from the Feany Laboratory at the Ann Romney Center for Neurologic Disease at Brigham and Women’s Hospital for assistance with developing the protocol. The author would also like to acknowledge the Pathology Core at the Dana-Farber Cancer Institute for their work in optimizing the 2F12 antibody concentration.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • John B. Sanderson
    • 1
  1. 1.The Warren Alpert Medical School of Brown UniversityProvidenceUSA

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