Miniaturized Checkerboard Assays to Measure Antibiotic Interactions

  • Melike Cokol-Cakmak
  • Murat CokolEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1939)


Drugs may have synergistic or antagonistic interactions when combined. Checkerboard assays, where two drugs are combined in many doses, allow sensitive measurement of drug interactions. Here, we describe a protocol to measure the pairwise interactions among three antibiotics, in duplicate, in 5 days, using only two 96-well microplates and standard laboratory equipment.

Key words

Drug interactions Checkerboard assay Drug synergy 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Faculty of Engineering and Natural SciencesSabanci UniversityTuzlaTurkey
  2. 2.Department of Molecular Biology and MicrobiologyTufts University School of MedicineBostonUSA
  3. 3.Laboratory of Systems PharmacologyHarvard Medical SchoolBostonUSA

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