Modeling FXS with Mouse Neural Progenitors

  • Ulla-Kaisa Peteri
  • Maija L. Castrén
Part of the Methods in Molecular Biology book series (MIMB, volume 1942)


The neurosphere assay is a widely used method to culture neural precursor cells (NPCs), which include mixed populations of neural stem and progenitor cells, from the mammalian central nervous system. Fmr1-knockout (KO) mice generated to model fragile X syndrome (FXS) recapitulate the major phenotype of FXS. Neurosphere differentiation of cortical progenitors derived from brains of Fmr1-KO mice has been shown to reflect disordered mechanisms during cortical development in FXS in vivo. The cellular composition of neurospheres is heterogeneous, but robust FXS-specific alterations can be identified when culturing conditions are kept constant.

Key words

Mouse Cell culture Brain Neurosphere Differentiation Neural progenitor 



This protocol was adapted from the method described by Johansson et al. [2] and our previously published paper [8]. This work was supported by Academy of Finland, Tuulikki and Sakari Sohlberg Foundation, and Arvo and Lea Ylppö Foundation.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Ulla-Kaisa Peteri
    • 1
  • Maija L. Castrén
    • 1
  1. 1.Faculty of Medicine, PhysiologyUniversity of HelsinkiHelsinkiFinland

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