Induction of Metabotropic Glutamate Receptor-Mediated Long-Term Depression in the Hippocampal Schaffer Collateral Pathway of Aging Rats

  • Kirstan E. Gimse
  • Kenneth O’Riordan
  • Corinna BurgerEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1941)


Scientific progress in the understanding of the molecular mechanisms of aging in the brain is essential for the identification of novel targets for the treatment and prevention of age-associated cognitive disorders. Electrophysiological analysis of synaptic plasticity using extracellular field recordings in rodent hippocampal slices is a well-established method for investigating molecular mechanisms of learning and memory. These methods can be applied to the study of aging in the brain by utilizing hippocampal slices from aged animals. However, this application can be challenging as it is difficult to ensure and maintain the health of slices originating from aged animals. The technique described in this chapter outlines the procedure for measuring metabotropic glutamate receptor-mediated long-term depression in hippocampal slices using extracellular field recordings and includes specific details for the application of this technique in the study of neuronal aging.

Key words

Metabotropic glutamate receptors Long-term depression Aging Hippocampus Synaptic plasticity Field recordings 



This work was supported by NIH R01AG048172-03 and NIH T32 GM081061 to Kirstan Gimse.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Kirstan E. Gimse
    • 1
  • Kenneth O’Riordan
    • 2
  • Corinna Burger
    • 1
    • 3
    Email author
  1. 1.Cellular and Molecular Pathology Graduate ProgramUniversity of Wisconsin-MadisonMadisonUSA
  2. 2.APC Microbiome IrelandUniversity College CorkCorkUK
  3. 3.Department of NeurologyUniversity of Wisconsin-MadisonMadisonUSA

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