Electrophysiological Investigation of Metabotropic Glutamate Receptor-Dependent Metaplasticity in the Hippocampus

  • Regina U. Hegemann
  • Wickliffe C. AbrahamEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1941)


Metabotropic glutamate receptors (mGluRs) are one of the major types of glutamatergic receptors contributing to synaptic plasticity mechanisms such as long-term potentiation (LTP) and long-term depression. Interestingly, activation of mGluRs alone can engage metaplastic mechanisms that create a new neuronal state, facilitating the induction and maintenance of future LTP. Here we describe typical methods used to investigate mGluR-induced metaplasticity in acute hippocampal slices. While this chapter focuses on in vitro field electrophysiological investigations, many of the principles can be applied to single-cell recordings as well as in vivo electrophysiology and indeed many types of metaplasticity phenomena.

Key words

Metaplasticity Synaptic plasticity Metabotropic glutamate receptors Long-term potentiation Long-term depression Hippocampus 



We wish to thank Dr. Bruce Mockett for his contribution to Fig. 1 and the University of Otago for financial support of R.H. Hegemann.


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Psychology, Brain Health Research Centre and Brain Research New ZealandUniversity of OtagoDunedinNew Zealand

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