Metabolomics Analysis of Glutamate Receptor Function

  • Nataliya E. ChornaEmail author
  • Anatoliy P. Chornyy
Part of the Methods in Molecular Biology book series (MIMB, volume 1941)


Metabolomics analysis provides a unique opportunity for comprehensive mapping of glutamatergic system function using high-throughput metabolite analysis and providing a perspective of the enzymatic activity, which cannot be obtained by other systems biology techniques.

Key words

Metabolomics Brain Glutamate Glutamate receptor Metabolites Gas chromatography Mass spectrometry 



This work was supported by INBRE-PR NIH Grant 8P20GM103475.


  1. 1.
    Vasilopoulou CG, Margarity M, Klapa MI (2016) Metabolomic analysis in brain research: opportunities and challenges. Front Physiol 7:183CrossRefGoogle Scholar
  2. 2.
    Moussa CE-H et al (2002) Effects of L-glutamate transport inhibition by a conformationally restricted glutamate analogue (2S,1'S,2'R)-2-(Carboxycyclopropyl)glycine (L-CCG III) on metabolism in brain tissue in vitro analysed by NMR spectroscopy. Neurochem Res 27(1):27–35CrossRefGoogle Scholar
  3. 3.
    Rae C et al (2006) A metabolomic approach to Ionotropic glutamate receptor subtype function: a nuclear magnetic resonance in vitro investigation. J Cereb Blood Flow Metab 26(8):1005–1017CrossRefGoogle Scholar
  4. 4.
    Kanani H, Chrysanthopoulos PK, Klapa MI (2008) Standardizing GC–MS metabolomics. J Chromatogr B 871(2):191–201CrossRefGoogle Scholar
  5. 5.
    Spijker S (2011) Dissection of rodent brain regions. In: Li KW (ed) Neuroproteomics. Humana Press, Totowa, NJ, pp 13–26CrossRefGoogle Scholar
  6. 6.
    Xia J et al (2015) MetaboAnalyst 3.0—making metabolomics more meaningful. Nucleic Acids Res 43(W1):W251–W257CrossRefGoogle Scholar
  7. 7.
    Sapcariu SC et al (2014) Simultaneous extraction of proteins and metabolites from cells in culture. MethodsX 1:74–80CrossRefGoogle Scholar
  8. 8.
    van den Berg RA et al (2006) Centering, scaling, and transformations: improving the biological information content of metabolomics data. BMC Genomics 7:142CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Biochemistry, School of MedicineUniversity of Puerto RicoSan JuanUSA
  2. 2.PR-INBRE Metabolomics Research Core, School of MedicineUniversity of Puerto RicoSan JuanUSA
  3. 3.High Performance Computing Facility, Central AdministrationUniversity of Puerto RicoSan JuanUSA

Personalised recommendations